喷雾干燥法制备1型糖尿病反义肽噬菌体壳聚糖微球疫苗

Preparation of Bacteriophage-Displayed Diabete Type 1 Vaccine in Chitosan Microspheres by Spray Drying

目的制备1型糖尿病反义肽噬菌体壳聚糖微球疫苗,并观察其热稳定性。方法采用水溶性壳聚糖、海藻糖、甘氨酸作为保护剂,通过喷雾干燥技术制备1型糖尿病反义肽噬菌体壳聚糖微球疫苗,扫描电镜下观察其形态及粒径大小,并检测入口温度及海藻糖浓度对壳聚糖微球疫苗活性的影响。将喷雾干燥样品置37℃放置6d,检测其热稳定性。结果通过喷雾干燥法制备的1型糖尿病反义肽噬菌体壳聚糖微球疫苗电镜下呈不规则的球形,粒径大小为20μm左右。喷雾干燥后样品的滴度与喷雾干燥前的疫苗原液相比均降低,且随着入口温度的升高而先升高后降低;喷雾干燥后的样品随着海藻糖浓度的增加,滴度也相应升高。与喷雾干燥前的疫苗原液相比,壳聚糖微球疫苗具有更好的热稳定性。结论已成功制备了1型糖尿病反义肽噬菌体壳聚糖微球疫苗,其具有良好的热稳定性。

Objective To prepare bacteriophage-displayed diabete type 1 vaccine in chitosan microspheres and observe its thermal stability. Methods Bacteriophage-displayed diabete type 1 vaccine in chitosan microspheres was prepared by spray drying using a protective agent composed of water-soluble chitosan, trehalose and glycine, then observed for shape and size by scanning electron microscopy. The effects of inlet temperature and trehalose concentration on activity of the prepared microsphere vaccine were observed. The vaccine sample after spray drying was stored at 37℃ for 6 d and observed for thermal stability. Results The prepared vaccine was in shape of irregular spheres at a mean size of about 20 μm under scanning electron microscope. The titer of vaccine af- ter spray drying was lower than that of bulk before spray drying, and increased with the increasing inlet temperature then decreased. However, the titer of vaccine sample after spray drying increased with the increasing trehalose concentration. Compared with the bulk before spray drying, the microsphere vaccine showed good thermal stability. Conclusion Bacteriophage-displayed diabete type 1 vaccine in chitosan microspheres was successfully prepared and showed good thermal stability.