人呼吸道合胞病毒裂解条件的初步探索

Preliminary Study on Condition for Lysis of Human Respiratory Syncytial Virus

目的对人呼吸道合胞病毒(HRSV)的裂解条件进行初步探索,为研制HRSV裂解纯化疫苗奠定基础。方法用不同的裂解剂,以不同浓度和温度对HRSV裂解不同时间,以HRSV的F及G蛋白为目标蛋白,对得到的蛋白进行还原蛋白电泳分析及Western blot鉴定,电镜观察验证裂解效果。结果1%浓度的TritonX-100在4℃作用90min,对HRSV的裂解效果优于其他裂解剂。电镜观察可见病毒样品呈云絮状裂解;Western blot分析表明,特异性条带为HRSV的F蛋白。结论已初步筛选出HRSV的裂解条件,其对HRSV的裂解效果较好。

Objective To explore the condition for lysis of human respiratory syncytial virus （HRSV） and lay a foundation of preparing purified HRSV cleavage vaccine. Methods HSRV was lysed with various lytic agents at various concentrations and temperatures for various time, then identified by reduced electrophoresis and Western blot and observed by electron microscopy. Results After lysis with 1% Triton X-100 at 4℃ for 90 rain, HRSV were nebular particles under electron microscope, and specific F protein band was proved by Western blot. Conclusion The condition for lysis of HRSV was preliminarily screened.