人工唾液浸泡对两种自酸蚀粘接剂粘接界面的影响

Effects of artificial saliva with bacteria on resin-dentin interfaces of two self-etching adhesives

目的探讨含细菌的人工唾液对两种自酸蚀粘接剂粘接界面纳米渗漏的影响。方法选用自酸蚀粘接剂Adper Prompt(AP)和Contax分别与正常牙本质粘接并制备微拉伸试件,之后将试件浸泡于含细菌的人工唾液中,分别在即刻、1周、1月、3月时取出进行硝酸银染色,用扫描电镜(SEM)观察粘接界面银沉积情况,并用NlH图像分析软件量化处理,以界面银沉积面积占断裂界面的百分比来评价界面纳米渗漏程度。结果粘接界面在即刻、1周、1月、3月时的银沉积面积百分比分别为AP:(17.00±5.40)%、(18.71±6.94)%、(22.81±5.60)%、(26.79±5.77)%;Contax:(11.90±5.17)%、(12.41±4.42)%、(14.73±3.46)%、(17.64±5.39)%。结论无论浸泡人工唾液与否,粘接试件都无法避免纳米渗漏的产生;含细菌的人工唾液浸泡会影响粘接的稳定性,其中以AP组的稳定性较差,浸泡三个月即表现出界面银渗漏程度的显著增加。

Objective To evaluate the effects of artificial saliva with bacteria storage on the nanoleakage within resin - dentin interfaces of two self - etching adhesives. Methods Adper Prompt （AP） and Contax were bonded with the normal dentin. Matchstick - shaped samples through the bond interfaces were sectioned and immersed in artifical saliva with bacteria. The resin - dentin interfaces of the the specimens were studied with scanning transmission electron microscopy （SEM） to identify the amount and distribution of silver uptake at 0 day , 1 week, 1 month and 3 months respectively after stained by silver nitrate. They were then subjected to image analysis by NIH Image PC. Leakage scores of each restoration were calculated as the percentage of the total area of the broken interface that was penetrated by silver. Results The ratios of the silver leakage of the resin - dentin interfaces at 0 day ,1 week,1 month and 3 month were as follows： AP（ 17.00 ± 5.40）% ,（18.71 ±6.94）% ,（22.81 ±5.60）% and （26.79±5.77）% ; Contax（11. 90 ±5.17）% ,（12.41 ±4.42）%, （14.73 ± 3.46）% and （17.64 ± 5.39 ）%. Conclusion All the bonding samples in this study could not resist nanoleakage. Artificial saliva with bacteria could impair the stability of bonding. After 3 months storage in artificial saliva with bacteria, the ratios of the silver leakage of AP increased significantly.