人促红细胞生成素对腹部开放伤大鼠经人工海水浸泡后所致急性肾损伤的保护作用

Protective effect of human erythropoietin on acute renal injury caused by abdomen open injury plus artificial seawater immersion in rats

目的:探讨人促红细胞生成素(EPO)对腹部开放伤加人工海水浸泡所致急性肾损伤大鼠的保护作用。方法:健康、雄性Wistar大鼠随机分成EPO预处理组、观察组、小剂量EPO救治组、大剂量EPO救治组,每组15只动物。腹部开放伤加人工海水(22℃)浸泡,制备急性肾损伤动物模型,检测各组实验动物血肌酐、尿素氮、肌酸激酶、肌酸激酶同工酶、TNF-α、IL-6、补体C3a、C反应蛋白、肾组织匀浆超氧化物歧化酶(SOD)水平,并观察肾脏病理学变化。结果:各组大鼠在腹部开放伤加海水浸泡3h后均发生了急性肾损伤改变,血清肌酐、尿素氮升高,但仍可存活;EPO预处理组与观察组及小/大剂量EPO救治组比较,大鼠血肌酐、尿素氮、肌酸激酶、肌酸激酶同工酶显著降低,TNF-α、IL-6、C3a、C反应蛋白等炎性因子水平明显降低,肾组织匀浆SOD明显升高,肾脏近曲小管坏死程度计分明显下降。而大、小剂量救治组间各项指标比较则无统计学差异(P>0.05)。结论:EPO预处理对腹部开放伤加海水浸泡所致急性肾损伤大鼠有保护作用。

Objective： To investigate the protective effect of erythropoietin （EPO） on the acute renal injuries caused by abdomen open injury plus seawater immersion in rats. Methods： Sixty healthy male Wistar rats （clean grade） were evenly randomized into four groups, namely, EPO pre-treatment group, observation group, low-dose EPO treatment group and high-dose EPO treatment group. Acute renal injury was induced by abdomen open injury plus artificial seawater immersion （22℃）. The serum creatine, BUN, creatine kinase, creatine kinase isoenzyme, TNF-α, IL-6, complement C3a, C-reactive protein, renal homogenate superoxide dismutase （SOD）, and the renal pathological changes were observed and compared between different groups. Results： Acute renal injury was observed in all groups 3 hours after abdomen open injury plus seawater immersion,with increased serum creatine and BUN, but the rats survived after treatment. The serum creatine, BUN,creatine kinase,and creatine kinase isoenzyme in EPO pre-treatment group were significantly lower than those of the other 3 groups; the levels of TNF-α,IL- 6,complement C3a,and C-reactive protein were also obviously decreased; the renal homogenate SOD was obviously increased; and the score of renal proximal tubule necrosis was obviously decreased. However, no significant differences were found between the high- and low-dose EPO groups concerning all the parameters （P〉0.05）. Conclusion： EPO pre-treatment has a protective effect on the acute renal injury induced by abdomen open injury plus seawater immersion in rats.