热休克蛋白70-1基因多态性与冠心病的关联性研究

Association Study of Heat Shock Protein 70-1 Gene Polymorphism and Coronary Heart Disease

目的:探讨热休克蛋白70-1(HSP70-1)基因多态性与冠心病的关系。方法:采用病例-对照1:1研究设计。选择湖北省三家综合性医院的217例冠心病患者作为病例组,同时选年龄、性别匹配的217例健康者作为对照组。采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法检测HSP70-1多态性。结果:HSP70-1基因各基因型及等位基因频率在两组中的分布差异有统计学意义。结论:HSP70-1基因多态性可能是冠心病的易感基因。

Objective To explore the association between gene polymorphism of heat shock protein 70-1 （HSP70-1） and the susceptibility to coronary heart disease. Methods A matched case-control study was performed. Two hundreds and seventeen patients with coronary heart disease were selected as case group from three general hospitals in Hubei province, and 217 healthy subjects with age and gender matched were simultaneously selected from the same geographic area as control. HSP70-1 genotypes were determined with polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP）. Results There were significant diferenees in the distribution of HSP70-1 genotypes and allele frequency between case group and control group. Conclusion HSP70-1 gene polymorphism may be one of genetic susceptibility factors of coronary heart disease.