反相高效液相色谱法测定油脂及饲料中BHA和BHT

Rapid determination of antioxidants BHA and BHT in oil and forage by revered-phase High Performance Liquid Chromatography

利用甲醇超声一次提取样品中的BHA、BHT，并使用反相HPLC测定含量。试验结果表明：校准曲线在1．0～50μg／mL范围内相关系数〉0．9990，不同类型的样品的相对标准偏差〈4．70％，回收率在90％～103％之间，样品最低检出限在0．002～0．005g／kg之间。该方法样品前处理步骤简单，经济环保，测定快速，结果准确，适用于油脂及饲料中BHA、BHT的快速检测。

The antioxidants BHA, BHT were extracted from oil and feed by methanol and determined by reversed-phase high performance liquid chromatography（RP-HPLC）. The results showed that linear correlation coefficients were above 0.9990 in the range of 1.0 - 50 μg/mL, RSDs of different samples were all less than 4.70%, the recovery rates were 90% - 103%, the lowest detection limit was 0.002 -0.005 g/kg. The method which is simple, rapid, accurate and economic is suitable for detecting BHA,BHT in oil and feed.