乌司他丁对脓毒症大鼠心组织基因表达的影响

Influence of Ulinstatin on gene expression of heart tissue in rats with sepsis

目的：基因水平上研究乌司他丁（Ulinastatin，UTI）的预防性应用对脓毒症晚期（24h）大鼠心脏组织基因表达的调控。方法：采用盲肠结扎穿孔术（cecal ligation puncture，CLP）制备脓毒症模型。Wistar大鼠30只．随机分为UTI组和对照组，每组15只，透射电镜观察术后24h心脏组织病理学改变，采用含有22523个大鼠基因cDNA克隆的表达谱基因芯片进行检测并分析术前1h预防性应用乌司他丁的脓毒症晚期大鼠心脏组织的基因表达，并以计算机软件筛选出差异表达的基因。结果：UTI组心脏组织病理学改变有所减轻。在22523条基因中，有差异表达的基因202条，下调基因主要涉及细胞信号转导、物质能量代谢、免疫反应、胰岛素样生长因子、细胞骨架蛋白、离子通道等，上调基因主要涉及细胞信号转导、物质能量代谢、应激反应、免疫反应、趋化因子、离子通道、癌基因等。结论：UTI减轻脓毒症晚期大鼠心脏的组织损害，具有一定的心脏保护效应．其机制可能涉及细胞信号转导、物质能量代谢、趋化因子、胰岛素样生长因子，凋亡等多方面。

Objective： To investigate the preventive effect of Ulinstatin on regulation of gene expression in rats with late sepsis（24 hours） at gene level. Methods： Cecal ligation puncture was adopted to set up the model.30 Wistar rats were selected and randomly divided into UTI group and control group, each of which has 15.The application of transmission electron microscope was to observe pathebiological change of heart tissue postoperative 24 hours. Gene chips containing 22523 rat-genes cDNA was applied to detect and analyze variation of gene expression of heart tissue in rats which were pretreated by UTI an hour prior to the operation,then software to sieve the differential expressive genes. Results; The heart pathobiological injury of UTI group was eased. There were 202 differential expression genes out of 22523.Down-regulated genes were mainly effered to cellular signal transduction, metabolism, immunoresponse, insulin-like growth factor, cytoskeletal protein, ion channel and so forth. Upregulated genes were chiefly concerned to cellular signal transduction, metaboli-stress response, immunoresponse, chemotactic factor, ion channel, onc-ogene and so on. Conclusion： UTI relieve heart tissue injury in rats with late sepsis and has some protective effect,its mechanism may refer to cellular signal transduction, metabolism, chemotactic factor, insulin-like growth factor, appotosis and so on.