摘要
目的对医院细菌室送检粪便标本中分离出的艰难梭菌,进行毒素基因以及耐药基因的初步分析。方法将细菌室收集到的112份粪便标本,经选择性厌氧培养,谷氨酸脱氢酶(GDH)以及API 20A生化条鉴定后,分离出12株艰难梭菌,分别采用PCR的方法检测tcdA和tcdB基因、二元毒素基因,克林霉素抗性基因(ermB)。结果12株艰难梭菌中8株为毒素基因阳性,其中tcdA+tcdB+为5株,占62.5%;tcdA-tcdB+为3株,占37.5%;二元毒素基因均为阴性;耐药基因ermB阳性为4株,占50%。结论艰难梭菌tcdA-tcdB+毒株所占比例增加,临床单独检测A毒素易造成漏检;艰难梭菌耐药现象值得关注。
Objective To analyze the toxin gene and drug resistance gene of Clostridium difficile isolated from the stool samples sent by hospitals. Methods A total of 12 strains of Clostridium difficile were isolated from 112 stool samples by selective anaerobic culture, glutamic acid dehydrogenase （GDH） and API 20A （bioMerieux） identification, then tcdA、tcdB genes, binary-toxin genes and clindamycin resistance gene （erm B ）were detected by PCR. Results Toxic Clostridium difficile was found in 8 out of 12 strains, 5 strains were tcdA ＋ tcdB＋ （ 62.5% ） ;3 strains were tcdA - tcdB ＋ （37.5%）; all the strains were negative for binary-toxin genes; and 4 out of 8 strains were positive for clindamycin resistance gene （ erm B ） （50%）. Conclusion Proportion of tcdA - tcdB ＋ Clostridium difficile increased, so single toxin A detection might lead to the missed diagnosis of Clostridium difficile-associated diarrhea （CDAD） patients; and more attention should be paid to the drug resistance of Clostridium difficile.
出处
《疾病监测》
CAS
2009年第3期193-195,共3页
Disease Surveillance
基金
卫生部传染病重大专项资助项目(NO.2008ZX10004-012)
