E-选择素基因rs3917422A和rs5355C位点多态性与哈尼族原发性高血压相关性研究

Association between E-Selectin gene rs5355C,rs3917422A polymorphism and essential Hypertension in Hani Minority

目的探讨E-选择素基因rs3917422A\C和rs5355C\T位点多态性与哈尼族原发性高血压的关系。方法采用PCR测序技术,对云南哈尼族172例原发性高血压患者和133例正常对照的E-选择素基因rs3917422A\C和rs5355C\T的错义突变进行检测。结果在哈尼族中未发现有rs3917422A多态存在,发现rs5355C基因有多态存在。rs5355C等位基因频率在原发性高血压组中为10.5%,正常对照组中为13.2%,两组之间无显著性差异(P=0.311,P>0.05)。结论哈尼族中E-选择素基因无rs3917422A多态基因存在,而多态性基因rs5355C基因频率分布是原发性高血压组低与正常对照组,其多态性与哈尼族原发性高血压成负相关,两组之间无显著性差异。

Objective To investigate the association between missense mutation in E-seleetin gene including rs3917422A ／ C rs5355C ／ T and essential hypertension in Hani minority.Methods E-selectin genotyped by sequence was performed in 172 essential hypertensive subjects and 133 normotensive subjects. Results The allele frequency of the rs5355C ／ T studied polymorphism in normotensive controls and essential hypertensive cohort in Hani population were 0. 132vs.0.105 .The frequency of rs5355C ／ T allele in the essential hypertensive group was not significantly higher than that in,the normotensive controls （ P = 0.311 P 〉 0.05）. No significant difference was found between the two groups. Conclusion The rs3917422A is not in the E-seleetin gene and the rs5355C is in the E-seleetin gene in Hani minorities. But missense mutation E-seleetin gene rs5355C Polymorphism are not significantly higher than tin the normotensive controls in Hani Minority.