摘要
研究了吖啶橙（AO）与人血白蛋白（HSA）相互作用所引起的共振瑞利散射（RRS）光谱，在pH7．87～8．10范围内，加入HSA导致AO共振瑞利散射剧烈增强，在λem=λex=525nm处，存在RRS增强峰，其散射光强度增加值与蛋白质的浓度呈线性关系，据此建立了一种测定蛋白质的RRS法，方法的线性范围为0～10．0mg／L，检出限8．1μg／L。对共振瑞利散射法（RRS）测定尿微量白蛋白的方法进行临床评估，旨在为尿微量白蛋白测定选择一种简便快速、准确和易推广的方法。95例糖尿病患者及50名对照均留取8h尿样本，用RRS和RIA法测定，并进行比较和分析。结果显示，RRS和RIA尿微量白蛋白测定方法均具有高精密度、高准确性，两种方法的结果明显相关。与RIA法比较，RRS法简便、快速，没有放射性污染，且仪器、试剂易得，比较适合临床常规实验室应用。
A new resonance rayleigh scattering（RRS） assay was presented in this paper. At the optimum pH =7.87 -8.10,the weak RRS of acridine orange （AO）can be greatly enhanced by the addition of proteins due to the interaction between protein and AO at λem = λex = 525 nm. A new quantitative determination method for proteins has been developed. The linear range for human serum albumin was 0 - 10.0 mg/L with detection limit of 8.1 μg/L. To evaluate the clinical effect of two methods for determination of urinary microalbumin with RRS and RIA, and to select a method which is simple, quick and accuracy,8 h urine was collected in 95 diabetic patients and 50 health controls, and determined by RRS and RIA. In conclusion, the two methods for determination of urinary microalbumin were precise, sensitivity and significantly correlated. However, RRS was more simple, quick, reliable and without contaminated of radioisotope compared with RIA. The method can be popularized which was suitable to be clinically used as a routine laboratory test.
出处
《应用化工》
CAS
CSCD
2009年第4期612-614,624,共4页
Applied Chemical Industry
基金
湖南省卫生厅科研项目资助(C2003-014)
