感染状态下肿瘤坏死因子和谷氨酰胺的变化

The variations of glutamine and TNF-α concentration in septic rats

目的:探讨感染不同时期内毒素、肿瘤坏死因子(TNF-α)和Gln浓度的变化。方法:将90只大鼠随机分为对照组(正常组)和盲肠结扎穿孔(CLP)的实验组。动物模型制作成功后6、12、24、48和72 h采取血浆、肝、骨骼肌和小肠,并检测血浆内毒素、TNF-α和Gln浓度以及肝、骨骼肌和小肠的Gln浓度。结果:在脓毒症大鼠中,血浆内毒素、TNF-α均于6 h开始升高,12 h显著升高,24 h达高峰,48和72 h显著下降;血浆中Gln 6～12 h升高,24～48 h维持高峰,72 h开始下降;肝内Gln浓度6～12 h升高,24 h显著升高至高峰,48和72 h显著下降,并低于正常水平;小肠、骨骼肌中Gln浓度6～12 h下降不显著,24 h以后显著下降,并低于正常水平。结论:在感染早期(6～12 h),血中内毒素和TNF-α浓度显著升高;血浆和肝内Gln浓度显著升高,小肠和骨骼肌中Gln浓度下降不明显。

Objective： This study was designed to investigate the variations of endotoxin, glutamine and TNF-α concentration in septic rats. Methods：A total of 90 adult male SD rats were divided as follows： control group （normal） ,experimental group （CLP）. They were killed on hour 6,12,24,48,72 after surgery. Plasma, liver, small intestine and skeletal muscle were collected to measure the concentrations of glutamine in plasma and tissues, the levels of endotoxin and TNF-α in plasma. Results ： In septic rats, plasma concentrations of endotoxin and TNF-α increased on hour 6, significantly increased on hour 12, attained the peal（ on hour 24, and decreased evidently on hour 48 and 72. Glutamine concentration in plasma elevated on hour 6 and 12, elevated significantly on hour 24 and 48, and decreased on hour 72. Glutamine concentration in liver increased on hour 6 - 12, increased significantly on hour 24, and decreased significantly on hour 48 and 72. Glutamine concentration in small intestine and skeletal muscle decreased on hour 6 and 12, and decreased significantly after 24 hour. Conclusion ： During the early stage of sepsis, the plasma levels of endotoxin and TNF-α are increased significantly, the glutamine concentration in plasma and liver is also increased significantly, however, it was decreased evidently in skel-etal muscle and small intestine.