超排卵对小鼠着床期雌、孕激素及其受体的影响及中药调节

Effect of superovulation on levels of estrogen,progesterone and their receptors in implantation period of rats and the regulation of chinese medicine

目的:观察补肾安胎方对超排卵小鼠阴栓阳性率、妊娠率、着床位点数和雌、孕激素及其受体水平的影响。方法:将180只小鼠随机分为正常组、模型组和中药组。模型组和中药组注射孕马血清促性腺激素(PMSG)和HCG建立小鼠超排模型后合笼,正常组注射等体积生理盐水后合笼,妊娠第1、2、5、8天留取血清或子宫及卵巢。观察每组小鼠的阴栓阳性率、妊娠第8天妊娠率和着床位点数;采用放免法检测各组小鼠雌、孕激素含量;采用免疫组化技术检测小鼠子宫雌、孕激素受体表达水平。结果:①模型组阴栓阳性率、妊娠率显著低于正常组(P<0.05),胚泡着床位点数显著高于正常组(P<0.05);中药治疗后阴栓阳性率、妊娠率显著高于模型组(P<0.05),胚泡着床位点数较模型组降低(P<0.05)。②妊娠第1、2天各组小鼠卵巢重量比较:模型组和中药组显著高于正常组(P<0.05),妊娠第1天中药组和模型组比较差异无统计学意义,妊娠第2天中药组显著低于模型组(P<0.05)。③血清雌、孕激素水平:妊娠第1、5、8天同时期3组血清雌激素水平比较差异无统计学意义,模型组孕激素水平显著高于正常组(P<0.05),中药治疗后孕激素水平降低,与正常组比较差异无统计学意义。④子宫内膜雌、孕激素受体表达:模型组雌、孕激素受体水平较正常组降低,中药治疗后较模型组升高。结论:补肾安胎方能提高超排卵小鼠阴栓率和妊娠率,调节超排卵小鼠的着床位点数,其机制可能与该方能改善甾体激素水平,调节子宫内膜雌、孕激素受体表达有关。

Objective： To observe the effect of Bushen＇antai recipe （BAR） on vaginal plug rate, pregnancy rate, the number of implantation site and the expression of estrogen, progesterone and their receptors in superovulation rats. Methods： 180 rats were divided into three groups randomly： control group, model group and traditional chinese medicine group （TCM group） . The rats in model group and TCM group were injected with pregnant mare serum gonadotrophin, while the rats in control group were injected with the same volume normal saline, uterus and ovary or serum of rats in three groups were collected on the first, second, fifth and eighth pregnancy day. Vaginal plug rate, pregnancy rate on eighth pregnancy day and the number of implantation site were recorded; the levels of estrogen, progesterone and their receptors were detected by radioimmunity and immunohistochemistry. Results： ①The vaginal plug rate and pregnancy rate in model group were respectively lower than those in normal group （ P 〈 0. 05 ）, while the number of implantation site reversed （ P 〈 0.05 ） . The vaginal plug rate and pregnancy rate in TCM group were higher than those in model group （ P 〈 0. 05 ）, while the number of implantation site reversed （P 〈 0. 05） . ②The ovarian weigbts of model group and TCM group were significantly higher than than of control group on the first and second pregnancy day （ P 〈 0. 05 ）, however, there was no difference between normal control group and TCM group on the first pregnancy day, the ovarian weight of TCM group was lower than that of model group on the second pregnancy day （P 〈 0. 05 ） . ③There was no difference in the level of estrogen between control group, model group and TCM group on the first, fifth and eighth pregnancy day, the level of progesterone in model group was higher than that in control group （P 〈 0. 05）, after the treatment of chinese medicine, the level of progesterone decreased, without significant difference compared with control group. ④The levels of estrogen receptor and progesterone receptor in model group were lower than those in control group. Conclusion ： BAR can improve vaginal plug rate and pregnancy rate of rats, regulate the number of implantation site, which is related to the change of steroid hormones levels.