复方连翘对Th细胞分化信号蛋白钙调神经磷酸酶转录表达的影响

Effects of the Compound Traditional Medicine Weeping Forsythia on Expression of Th Differentiation Signaling Transduction Protein Calcineurin

【目的】观察中药复方连翘对T淋巴细胞活化信号蛋白CaN转录表达变化,探讨其免疫调控机制。【方法】用含药血清培养鸡脾T淋巴细胞并以OVA和ConA体外干预诱导;利用半定量RT-PCR和Western-blotting技术检测CaN mRNA及其蛋白的表达。【结果】发现信号蛋白CaN转录、表达在0.1%OVA的情况下无血清组和正常血清组高于其它实验组,与10%OVA相比差异显著(P<0.05)。0.1%OVA浓度下,抗病毒口服液与复方连翘血清组的CaN表达均有明显降低,但两者之间降低程度无显著差别;复方连翘血清组在10%OVA条件下的CaN转录表达,与无血清组比较,有显著升高(P<0.05)。【结论】复方连翘可能通过调节信号转导通路第一个接头蛋白CaN的转录表达来影响Th的分化进而实现对机体的免疫调控,同时也表明复方连翘通过双向调节、多环节、多途径实现的对机体免疫调节作用。

[Objective] The objective of this experiment is to explore the mechanism of the compound traditional medicine weeping forsythia regulating immunity by testing the expression of the differentiation signaling transduction protein, calcineurin. [Method] To prepare the compound weeping forsythia prescription serum, the Ova and ConA were used as antigen in induction and T cell as presenting cell, the T cells were inlervening cultured for 24 hours, and then the expression of calcineurin was examined by RT- PCR and Western blot. [Result] The expression of CaN mRNA was elevated in the 0.1% Ova groups comparing with normal serum and serum-free group. The 0.1% Ova groups were significally different with the 10% Ova groups （P〈0.01）. The protein expression of CaN was significantly down regulated in compound weeping forsythia serum groups with Western blot detecting, but there was no significant difference between the two groups at concentration of 0.1% Ova. The CaN expression of the compound weeping forsythia serum group was elevated obviously compared with the group without serum （P〈0.05）. [Conclusion] The compound weeping forsythia has provided an immunomodulation by transduction of regulatory signal, and by realizing many aspects and double direction regulation. The results showed that the compound weeping forsythia has more referential values of the selection medication on deficiency syndrome and excess syndrome.