21种杀虫剂和3种植物次生物质对杨扇舟蛾各组织谷胱甘肽S-转移酶的抑制作用

Inhibition of glutathione S-transferase activity by twenty-one insecticides and three allelochemicals in various tissues of Clostera anachoreta(Fabricius)(Lepidoptera:Notodontidae)

为了比较杨扇舟蛾Clostera anachoreta(Fabricius)各组织谷胱甘肽S-转移酶(GSTs)的差异,利用分光光度酶动力学的方法,研究了21种杀虫剂和3种植物次生物质对杨扇舟蛾4个组织(中肠、脂肪体、头部和体壁)GSTs活性的体外影响。结果表明:21种杀虫剂和3种植物次生物质对杨扇舟蛾4个组织GSTs活性的抑制作用不同。毒死蜱、氟虫腈、槲皮素和单宁酸对于杨扇舟蛾头GSTs活性抑制作用最强;槲皮素和单宁酸对中肠GSTs活性的抑制作用最强;单宁酸对脂肪体GSTs活性的抑制作用最强;辛硫磷、高效氯氟氰菊酯、溴氰菊酯和硫丹对皮GSTs活性的抑制作用最强。杨扇舟蛾4个组织GSTs对杀虫剂和植物次生物质敏感性存在的这种差异,可能是由于其在同工酶组成上的差异造成的。

The inhibition of glutathione S-transferase （GSTs） activity in 4 tissues （ midgut, fat body, head and integument） of Clostera anachoreta （Fabricius） by 21 insecticides and 3 allelochemicals was conducted using the biochemistry methods in order to compare the difference of GSTs in various tissues. The results showed that the inhibition of GST activity of different tissues by 21 insecticides and 3 allelochemicals was different. For GST activity in the head, chlorpyrifos, fipronil, quercetin and tannic acid were the best inhibitors tested; while in midguts, quercetin and tannic acid were the most potent inhibitors tested; for GST activity in the fat body, the inhibitory effect of tannic acid was the highest, and the inhibitory effects of phoxim, lambda-cyhalothrin, deltamethrin and endosulfan were the highest for GST activity in the integument. The results suggest that the difference in the sensitivity of GSTs to 21 insecticides and 3 allelochemicals in different tissues of C. anachoreta might be due to the quantitative difference in isoezyme composition.