抵抗素对内皮细胞活性氧生成及AMPK磷酸化水平的影响

Effects of resistin on the ROS production and AMPK phosphorylation level

目的探讨抵抗素对内皮细胞功能影响的作用机制。方法体外培养人脐静脉内皮细胞,50、100 ng/ml抵抗素干预24 h,分别检测活性氧(ROS)生成和腺苷酸活化蛋白激酶(AMPK)磷酸化水平,并应用AICAR激活AMPK观察其对内皮ROS生成的影响。结果50、100 ng/ml抵抗素作用组与对照组相比,内皮细胞AMPK磷酸化水平明显下降,而ROS生成无明显改变;50 ng/ml抵抗素作用组加用AICAR干预后,AMPK磷酸化水平显著增高,同时伴ROS生成显著降低。结论抵抗素可影响内皮细胞功能,其机制可能通过抑制内皮细胞AMPK的磷酸化水平,参与内皮细胞炎症过程的调节,激活内皮AMPK,减少ROS生成,对内皮损伤起保护性作用。

Objective To investigate the mechanism of resistin on the function of endothelial cells. Methods Human umbilical vein endothelial cells（HUVECs） were cultured in vitro. Then treated with recombinant human resistin （50,100 ng/ml） for 24 hours. Reactive oxidative species（OS） production and AMP-activated protein kinase （AMPK） phosphorylation level were measured. AICAR was used for observing the role of AMPK in ROS production in HUVECs. Results The AMPK phosphorylation level in HUVECs decreased obviously after treated by 50 and 100 ng/ml resistin. Addition of AIC-AR to 50 ng/ml resistin group led to significant increase of AMPK phosphorylation level and decrease of ROS production. Conclusions Resistin can influence the the function of endothelial cells ,the mechanism maybe correlated to the inhibition of AMPK phosphorylation level and reduction of ROS production.