阿托伐他汀对脂多糖刺激下PBMC分泌TNFα-、IL-1β的影响

Effect of atorvastatin on TNF-α and IL-1β in peripheral blood monocytesstimulated by LPS

目的观察阿托伐他汀对脂多糖(LPS)刺激下外周血单核细胞(PBMC)分泌TNF-α、IL-1β的影响。方法Ficoll密度梯度离心法分离健康人外周血单个核细胞,不同浓度阿托伐他汀预处理2 h,而后加入10 ng/ml LPS共同培养24 h,采用酶联免疫吸附测定法(ELISA)检测单核细胞培养上清液肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的水平。结果1μmol/L阿托伐他汀减少LPS刺激下PBMC分泌TNF-α53.3%、IL-1β35.4%,10μmol/L阿托伐他汀减少TNF-α82.0%、IL-1β65.6%。结论阿托伐他汀能有效抑制LPS刺激下PBMC TNF-α及IL-1β的分泌。

Objective To observe the level of tumour necrosis factor alpha （TNF-α） and interleukin-1β （IL-1β） in human peripheral blood monocytes （PBMC） which were stimulated by LPS, and the effects of atorvastatin on it. Methods PBMCs were isolated from healthy persons by Ficoll density gradient separation and treated with atorvastatin （ 1 μmoL/L or 10 μmmol/L） for 2 hours, then LPS was added to co-culture for 1 day. TNF-α and IL-1β concentration was measured by enzyme-linked immunosorbent assay （ELISA）. Result Ceils treated with 1 μmoL/L atorvastatin showed reduced TNF- α 53.3%, IL-1β 35.4%, and 10 μmoL/L reduced TNF-α 82.0%, IL-1β 65.6%. Conclusion Atorvastatin can reduce the level of TNF-α and IL-1β in human PBMC stimulated by LPS.