邻苯二甲酸二丁酯亚慢性染毒对大鼠睾丸Insl3 mRNA表达的影响

Effects of Di-n-butyl Phthalate on Testicular Insl3 mRNA Expression in Pubertal Male Rats

背景与目的:观察邻苯二甲酸二丁酯(DBP)亚慢性染毒对青春期大鼠睾丸间质细胞功能标志物胰岛素样因子3(Insl3)mRNA表达水平的影响。材料与方法:青春期健康雄性SD大鼠72只,随机分为3组:溶剂对照组(玉米油),DBP 50 mg/kg和250 mg/kg剂量的染毒组,每组24只。隔日染毒,等体积灌胃,连续染毒90 d。分别于染毒后30、60和90 d各组随机选取8只大鼠处死。取睾丸、附睾、心、肝、脾、肾称重并计算脏器系数,放免法检测血清睾酮含量,实时荧光定量PCR法检测睾丸Insl3 mRNA表达。结果:DBP 250 mg/kg组染毒90 d肝脏系数显著大于对照组(P<0.05);50 mg/kg组染毒60 d大鼠血清睾酮水平较对照组显著升高(P<0.05),而250 mg/kg组比50 mg/kg组显著下降(P<0.01);各染毒组大鼠睾丸Insl3 mRNA表达水平较对照组显著下调(P<0.01),染毒30 d、60 d时DBP 250 mg/kg剂量组较DBP 50 mg/kg组显著下调(P<0.05),各染毒组睾丸Insl3 mRNA表达水平均随处理时间延长先下调而后上调,60 d较30 d显著降低(P<0.05),90 d较60 d显著升高(P<0.01)。结论:青春期大鼠DBP亚慢性暴露可干扰血清睾酮及睾丸Insl3 mRNA表达水平,对肝脏可能有潜在毒性。

BACKGROUND AND AIM： To explore the effects of subchronic exposure to di-n-butyl phthalate（DBP） on testicular insulin-like factor 3 （Insl3） mRNA expression in pubertal male rats. MATERIALS AND METHODS： A total of 72 male Sprague-Dawley rats at the age of 4- 5 weeks were equally randomized into 3 groups, to receive by gavage 0,50 or 250 mg/kg of DBP on alternate day for 90 days. Body, testis , epididymis and main organs were weighed. Serum testosterone, and mRNA expression of Insl3 in the testis were measured. RESULTS： There was an increase of the liver/ body ratio in 250 mg/kg group after 90 days treatment（P〈0.05） .Compared with the control, an increase in serum testosterone was found in the 50 mg/kg group（P 〈 0.05）, but there was a reduction in 250 mg/kg group compared with the 50 mg/kg group（P 〈 0.01）after 60 days exposure. The mRNA expressions of Insl3 were significantly down regulated in all dosage groups（P〈0.01）, more significantly in 250 mg/kg group than in 50 mg/kg group（P〈0.05） CONCLUSION： Pubertal male rats subchronically exposed to DBP could induce changes in serum testosterone and testicular Insl3 mRNA expression,and could have potential adverse effect on the liver.