摘要
背景与目的:观察邻苯二甲酸二丁酯(DBP)亚慢性染毒对青春期大鼠睾丸间质细胞功能标志物胰岛素样因子3(Insl3)mRNA表达水平的影响。材料与方法:青春期健康雄性SD大鼠72只,随机分为3组:溶剂对照组(玉米油),DBP 50 mg/kg和250 mg/kg剂量的染毒组,每组24只。隔日染毒,等体积灌胃,连续染毒90 d。分别于染毒后30、60和90 d各组随机选取8只大鼠处死。取睾丸、附睾、心、肝、脾、肾称重并计算脏器系数,放免法检测血清睾酮含量,实时荧光定量PCR法检测睾丸Insl3 mRNA表达。结果:DBP 250 mg/kg组染毒90 d肝脏系数显著大于对照组(P<0.05);50 mg/kg组染毒60 d大鼠血清睾酮水平较对照组显著升高(P<0.05),而250 mg/kg组比50 mg/kg组显著下降(P<0.01);各染毒组大鼠睾丸Insl3 mRNA表达水平较对照组显著下调(P<0.01),染毒30 d、60 d时DBP 250 mg/kg剂量组较DBP 50 mg/kg组显著下调(P<0.05),各染毒组睾丸Insl3 mRNA表达水平均随处理时间延长先下调而后上调,60 d较30 d显著降低(P<0.05),90 d较60 d显著升高(P<0.01)。结论:青春期大鼠DBP亚慢性暴露可干扰血清睾酮及睾丸Insl3 mRNA表达水平,对肝脏可能有潜在毒性。
BACKGROUND AND AIM: To explore the effects of subchronic exposure to di-n-butyl phthalate(DBP) on testicular insulin-like factor 3 (Insl3) mRNA expression in pubertal male rats. MATERIALS AND METHODS: A total of 72 male Sprague-Dawley rats at the age of 4- 5 weeks were equally randomized into 3 groups, to receive by gavage 0,50 or 250 mg/kg of DBP on alternate day for 90 days. Body, testis , epididymis and main organs were weighed. Serum testosterone, and mRNA expression of Insl3 in the testis were measured. RESULTS: There was an increase of the liver/ body ratio in 250 mg/kg group after 90 days treatment(P〈0.05) .Compared with the control, an increase in serum testosterone was found in the 50 mg/kg group(P 〈 0.05), but there was a reduction in 250 mg/kg group compared with the 50 mg/kg group(P 〈 0.01)after 60 days exposure. The mRNA expressions of Insl3 were significantly down regulated in all dosage groups(P〈0.01), more significantly in 250 mg/kg group than in 50 mg/kg group(P〈0.05) CONCLUSION: Pubertal male rats subchronically exposed to DBP could induce changes in serum testosterone and testicular Insl3 mRNA expression,and could have potential adverse effect on the liver.
出处
《癌变.畸变.突变》
CAS
CSCD
2009年第3期161-164,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家科技部西部引导项目(2003BA869C)
国家自然科学基金重点项目(30630056)
重庆市重大科技专项(CSTC2006AA7003)