摘要
背景与目的:初步探讨肺上皮样血管内皮瘤细胞遗传学改变的特点。材料与方法:提取2例肺上皮样血管内皮瘤石蜡包埋的肿瘤组织DNA,利用微阵列比较基因组杂交(array-CGH)技术检测瘤组织DNA拷贝数缺失和扩增片段。结果:2例肺上皮样血管内皮瘤DNA拷贝数改变有诸多类似之处,且均以多条染色体DNA小片段(小于10 Mb)扩增为主,很少有缺失区域;具体涉及的基因分别为150个和556个,共同改变的基因为77个。结论:肺上皮样血管内皮瘤细胞遗传学改变可能主要以小片段DNA扩增为特点,该特点可以诠释此肿瘤的交界性或低度恶性临床病理特征。
BACKGROUND AND AIM: To study the characteristics of cytogenetic aherations of lung epithelioid hemangioendothelioma. MATERIALS AND METHODS: Whole DNA was isolated from two formalin-fixed and paraffin-embedded tissues of lung epithelioid hemangioendothelioma. The DNA copy number changes were measured by array-comparative genomic hybridization(array-CGH) RESULTS: Two epithelioid hemangioendotheliomas showed many similar DNA copy number changes. Gains of small fragments( 〈 10 Mb) were the most frequent change while deletions were infrequent.There were 150 and 556 genes involved with 77 sharing common changes. CONCLUSION: Amplifications of small fragments of DNA maybe the main cytogenetic alteration in lung epithelioid hemangioendothelioma, which may explain that epithelioid hemangioendothelioma is a low-grade malignant tumor.
出处
《癌变.畸变.突变》
CAS
CSCD
2009年第3期185-188,共4页
Carcinogenesis,Teratogenesis & Mutagenesis