乳腺癌组织中Maspin蛋白表达与maspin基因启动子甲基化关系的研究

Analysis of Maspin Protein Expression and maspin Gene Promoter Methylation in Breast Cancer Tissue

背景与目的:探讨乳腺癌与maspin基因失活之间的关系。材料与方法:采用免疫组织化学方法检测30例乳腺癌患者癌组织、癌近旁组织和癌远旁组织中Maspin蛋白的表达情况,用亚硫酸氢钠测序法检测乳腺癌组织maspin启动子CpG岛的甲基化情况。结果:乳腺癌组织中的Maspin蛋白表达明显低于癌近旁组织(P<0.05)和癌远旁组织(P<0.01),乳腺癌组织maspin基因启动子CpG岛甲基化率为98.72%。结论:乳腺癌的发生与maspin基因失活关系密切,启动子异常甲基化可能是maspin基因在乳腺癌中失活的重要途径。

BACKGROUND AND AIM： To investigate the relationship between the inactivation of maspin gene and breast cancer. MATERIALS AND METHODS： The expression of Maspin protein in breast cancer tissue, adjacent non-tumor tissue, and distal normal tissue from 30 cases were detected by immunohistochemical method. The CpG islands methylation states of maspin promoter in breast cancer tissue were studied by bisulfite sequencing PCR （BSP）. RESULTS： The protein expression levels of maspin in breast cancer were significantly lower than that in adjacent non-tumor tissue （P 〈0.05）, and in distal normal tissue （P 〈 0.01）. The methylation incidence of maspin gene promoter CpG islands in breast cancer tissue was 98.72%. CONCLUSION： These findings suggest a close correlation between the inactivation of maspin gene and breast carcinogenesis. The aberrant methylation of promoter maybe is important mechanism of maspin gene inactivation in human breast carcinogenesis.