硫酸铍对人胚肺成纤维细胞的细胞毒性和遗传毒性

Cytotoxicity and Genotoxicity of Beryllium Sulfate to Human Embryonic Lung Fibroblast

背景与目的:探讨硫酸铍对体外培养的人胚肺成纤维细胞(HEL-I)的细胞毒性和遗传毒性。材料与方法:用不同浓度的硫酸铍(0.2、2.0、20.0、100.0、200.0μmol/L)作用HEL_I细胞24 h,采用MTT法测定细胞存活情况,用单细胞凝胶电泳(SCGE)和微核实验测定硫酸铍对HEL_I细胞遗传损伤的情况。结果:随着硫酸铍浓度的增加,HEL-I细胞的存活率呈下降趋势,硫酸铍浓度为100.0μmol/L和200.0μmol/L时,存活细胞数低于空白对照组(P<0.05);在2.0～100.0μmol/L浓度范围内,硫酸铍均可诱发HEL-I细胞出现DNA损伤和微核率升高(P<0.05)。结论:硫酸铍对HEL-I细胞具有明显细胞毒性和遗传毒性。

BACKGROUND AND AIM： To explore the cytotoxicity and genotoxicity of beryllium sulfate to human embryonic lung fibroblast （HEL-I） in vitro .MATERIALS AND METHODS： HEL-I cells were cultured with different concentrations of beryllium sulfate for 24 h, cell survival rate was measured by MTT test, cell genetic damage was assessed by single-cell gel electrophoresis and micronucleus test . RESULTS： The survival rate of HEL-I cells decreased along with increasing concentrations of the beryllium sulfate When the concentration of beryllium sulfate reached 100.0 μmol/ L and 200.0 μmol/ L, the survival rates of HEL-I cells were lower than control group. And beryllium sulfate caused DNA damage and micronucleus rate elevations in HEL-I cells at concentrations between 2.0 μmol/L and 100.0 μmol/L （P 〈 0.05）. CONCLUSION： Beryllium sulfate had obvious cytotoxicity and genotoxicity in HEL-I ceils.