B16黑色素瘤皮内移植瘤模型的建立

Intradermal Model of B16 Melanoma in Mice

背景与目的:建立B16黑色素瘤细胞(简称B16细胞)皮内移植瘤模型。材料与方法:制备商品源B16细胞悬液,按每只C57BL鼠接种0.1 ml(3×105个)B16细胞于后肢外侧皮内;分离、培养移植瘤源B16细胞,倒置显微镜下观察细胞形态。结果:移植瘤出现时间为(9.6±1.2)d,致瘤率为100%;皮内注射B16细胞18 d内,肿瘤形态呈圆形或类圆形,边缘清楚;移植瘤源原代B16细胞多为圆形、类圆形或短梭形,商品源B16细胞为梭形或不规则形;HE染色表明移植瘤内有大量B16细胞。结论:B16细胞皮内移植瘤模型易于对肿瘤生长的观察和测量,是早、中期肿瘤研究的合适模型之一。

BACKGROUND AND AIM： To establish an intradermal model with transplanting melanoma B16 cells in C57BL mice. MATERIALS AND METHODS： commercially-acquired 3×10^6 B16 cells in 0.1 ml were inoculated intradermally in the right hindlimb in C57BL mice. BI6 cells from tumors were isolated, cultured and examined. RESULTS： Tumor formation time was found to be （9.6±1.2）d and tumor formation rate was 100%. Within 18 d, tumors was round or round-like shape with distinct borders. Commercially-acquired B16 cells were mostly spindle or irregular whilst B16 cells from tumors were round or round-like or short-spindle. HE staining showed that there were a large number of B16 cells in the transplanted tumors. CONCLUSION： Intradermal tumor model of B16 cells in C57BL mice is helpful to monitor tumor growth in early and middle stages and is one of ideal models in tumor studies.