辐射抗性小鼠与其亲代差异基因的表达序列标签

Expressed Sequence Tags of Differential Genes in the Radioresistant Mice and Their Parental Mice

目的寻找IRM-2小鼠体内存在的与辐射抗性相关的基因。方法提取IRM-2小鼠和其亲代小鼠脾细胞总RNA,利用mRNA差异显示技术挑选差异表达片段,对每一条差异带进行特异PCR扩增、克隆和测序。结果获得IRM-2小鼠独有而亲代小鼠均无的cDNA差异带75条,经测序获得52条差异cDNA序列。与GenBank基因库同源性比较,得到21条与小鼠已知基因不同源的表达序列标签(EST)。结论21条EST提示IRM-2小鼠体内可能存在辐射抗性相关基因,为进一步分离和鉴定辐射抗性相关基因奠定了基础。

Objective To explore radioresistance correlative genes in IRM-2 inbred mouse. Methods The total RNA was extracted from spleen cells of IRM-2 and their parent 615 and ICR/JCL mouse. The mRNA differential display technique was used to analyze gene expression differences. Each differential bands were amplified by PCR,cloned and sequenced. Results There were 75 differential expression bands appearing in IRM-2 mouse but not in 615 and ICP/JCL mouse. Fifty-two pieces of cDNA sequences were got by sequencing. Twenty-one expressed sequence tags（EST） that were not the same as known mice genes were found and registered by comparing with GenBank database. Conclusion Twenty-one EST denote that radioresistance correlative genes may be in IRM-2 mouse, which have laid a foundation for isolating and identifying radioresistance correlative genes in further study.