基因功能研究策略在病原真菌中的应用

Application of Research Strategy for Gene Function to Entomopathogenic Fungi

昆虫病原真菌是病原真菌中一个大的类群,寄主达5目24科200余种昆虫。以昆虫病原真菌为主的杀虫剂在害虫的生物防治中起着重要的作用,其致病机制一直被人们广泛的研究。随着后基因组时代的到来,大规模基因的功能研究正成为热点,为进一步阐明病原真菌致病机理奠定了基础,因此,基因功能的分析方法也日趋成熟和多样化。对RNA干涉、基因敲除、生物信息学等基因功能分析策略的特异性和优缺点及其在病原真菌中的应用进行了综述。

Entomopathogenic fungi are a large group of pathogenic fungi, and include 200 species of host insect in 24 families of 5 orders. Because of effective and environmental friend control on insect pests, the entomopathogenic fungi have been extensively studied. With the coming of post-genomic era, large-scale study of gene function is becoming a hot spot, which has laid a foundation for further clarification for the pathogenic mechanism of pathogenic fungi. So analysis methods of gene function are becoming increasingly sophisticated and diverse. The paper reviews RNA interference, gene knock-out, and bioinformatics analysis, the advantages and disadvantages of the strategies, and their application in the pathogenic fungi.