替米沙坦对自发性高血压大鼠肌肉组织中PPARγ和PPARδ表达的影响

Effect of Telmisartan on the Protein Expression of PPARγ and PPARδ in Skeletal Muscle Tissues from Spontaneous Hypertensive Rats

目的观察替米沙坦对自发性高血压大鼠(SHR)肌肉组织和大鼠成肌细胞系L6细胞过氧化物酶体增殖激活受体(PPARγ、PPARδ)表达的影响,探讨替米沙坦影响糖代谢的可能机制。方法12只8周龄SHR分为对照组和替米沙坦干预组,经12周喂养后测鼠尾收缩压,取大腿肌肉组织用Westernblot检测PPARγ、PPARδ和血管紧张素Ⅱ1型受体(AT1R)表达;L6细胞分为对照组和替米沙坦组,干预24h后收集细胞用Westernblot检测PPARγ、PPARδ和AT1R表达。结果SHR替米沙坦组鼠尾收缩压较对照组明显下降(P<0.01),肌肉组织PPARγ、PPARδ表达增加(P<0.05);L6细胞替米沙坦组PPARγ、PPARδ的表达也增加(P<0.05和P<0.01)。结论替米沙坦除有降压作用外尚能促进肌肉组织中PPARγ和PPARδ的表达,这可能与替米沙坦改善糖代谢有关。

Objective To study the effect of telmisartan on the protein expression of peroxisome proliferator activated receptor γ and δ（PPARγ/PPARδ） in skeletal muscle tissues from spontaneous hypertensive rats （SHR） and in L6 skeletal myoblast. Methods Twelve 8-week-old male SHR were divided into normal diet group and normal diet plus telmisartan group. After 12-week admiru＇stration,tail-cuff systolic blood pressures were measured and the protein expression of PPARγ/PPARδ and angiotensin Ⅱ type 1 receptor （AT1R） from skeletal muscle tissues were detected by Western blot. L6 cells were treated with telmisartan or blank for 24 hours, and then cells were collected for measurement of protein expression of PPARγ/PPARδ and AT1R by Westem blot. Results Tail-cuff systolic blood pressures were significantly decreased in telmisartan group （P 〈 0.05 ）, while the expressions of PPARγ/and PPARδ in skeletal muscle tissues significantly increased （P 〈 0.05 ） compared with the control group. Telmisartan significantly increased the protein expressions of PPARγ/（P 〈 0.05 ） and PPARδ（P 〈 0.01 ） in L6 cells. Conclusion In addition to the role of blood pressure control, telmisartan can also increase the protein expressions of PPARγand PPARδ in the skeletal muscle of SHR, which would be implicated in the improvement of glycometabolism induced by telmisartan.