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FB1诱导肝癌细胞凋亡FasL表达的变化

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摘要 目的：了解不同浓度富马毒素（FB1）诱导肝癌细胞产生凋亡时FasLmRNA表达的变化方法：采用流式细胞术检测细胞的凋亡；荧光定量RT-PCR测定细胞FasLmRNA表达的水平。结果：5—40μmol／LFB1实验组均诱导肝癌细胞出现凋亡，经流式细胞仪检测凋亡率与对照组相比差异有显著性（P〈0．05）。荧光定量PCR检测结果显示，各实验组FasLmRNA与对照组相比表达均有一定程度降低，差异有显著性（P〈0．05）。结论：一定浓度的FB1可诱导肝癌细胞凋亡；各实验组FasLmRNA均无表达的增加并且降低。
作者 章亚倞 罗萍 陈建平
机构地区 成都中医药大学医学技术学院形态教研室 四川大学基础医学院与法医学院寄生虫学教研室
出处 《四川省卫生管理干部学院学报》 2009年第1期16-18,共3页 Journal of Sichuan Continuing Education College of Medical Sciences
关键词 富马毒素 肝癌细胞 细胞凋亡 FASL
分类号 R363 [医药卫生—病理学]
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参考文献8

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二级参考文献30

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四川省卫生管理干部学院学报
2009年 第1期

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