摘要
利用ISSR标记对59份山楂材料进行了遗传多样性分析。从51条引物中筛选出15条,用于山楂的ISSR扩增,共扩增出122条带,其中多态性条带113条,多态性为92.62%。根据ISSR扩增结果,利用DPS软件进行分析,通过UPGMA对59份材料进行聚类分析,认为伏山楂为山楂属的一个独立种,与山楂亲缘关系较近。同时,探讨了我国北方分布的山楂属植物的亲缘关系。
Fifty-nine hawthorn germplasm were used as materials for analyzing their genome polymorphism by ISSR markers. 15 primers selected from 51 primers were used for 1SSR amplification. A total of 122 bands were generated, of which 113 bands were polymorphic bands（the percentage of polymorphic band, PPB = 92.62 % ）. According to the results of ISSR amplification, which were analyzed to Jaccard similarity coefficient by DPS software, the genetic relationship of hawthorn accessions were analyzed and 59 hawthorn were classified on molecular level by clustered analysis, which provided reference for research in hawthorn. The results showed that C. brettschnederi is a species of Crateagus, which has a closer similarity with C. pinnatifida. At the same time, the relationship of Crateagus in Northern China was investigated.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2009年第2期164-167,共4页
Journal of Jilin Agricultural University
基金
吉林省科技发展计划项目(20060224)
关键词
伏山楂
ISSR
聚类分析
遗传多样性
C. brettschnederi
ISSR
cluster analysis
genetic diversity
