猪脱钙骨基质作为组织工程人工肋骨支架的可行性研究

Feasibility study on DBM as the tissue engineering artificial rib scaffold

目的评估异体脱钙骨基质作为组织工程化人工肋骨支架的可行性,为今后全胸壁缺损的骨性修复提供理论基础和实验依据。方法通过Micro-CT、扫描电镜观察猪脱钙骨基质的大体三维结构,通过细胞黏附实验观察猪脱钙骨基质对于异种骨髓基质干细胞的黏附情况,最后通过比较正常人肋骨与猪脱钙骨基质的力学性能评估其作为组织工程人工肋骨支架的可行性。结果Micro-CT和扫描电镜示猪脱钙骨基质具有良好的孔隙率和互相连通的孔径结构。细胞黏附实验显示细胞在猪脱钙骨基质表面生长良好。力学测试显示猪脱钙骨基质具有良好的力学性能,与人正常肋骨相比差异无统计学意义(P>0.05),因此可以用于修复具有弧度的肋骨缺损。结论猪脱钙骨基质可以作为组织工程人工肋骨的支架。

Objective To evaluate the feasibility of allogeneic demineralized bone matrix（DBM） as the tissue engineering rib scaffold. Methods The gross 3D structure of DBM was measured by Micro-CT and scanning electron microscope. The adhesion and proliferation of bone marrow stromal cells （BMSC） incubated on the DBM were observed by adhesion and proliferation experiment. The mechanical intensity was evaluated by three-point bending test. Results The imaging of Micro-CT and scanning electron microscopy demonstrated that the DBM had good porosity and the pores connected each other. The result of adhesion experiment showed that the cell inculated on the DBM grew well. The mechanical test showed that the mechanical properties of DBM were almost as good as the normal ribs without statistical difference. Conclusion The DBM is a suitable scaffold for tissue engineering artificial rib.