SYBR~ Green Ⅰ实时PCR检测转Cry1Ab/c基因水稻

Detection for Bt transgenic rice with SYBR~ Green Ⅰ real time PCR

为了建立转基因产品新型检测技术,采用SYBR Green Ⅰ实时PCR技术和三对特异引物,检测抗虫转基因水稻外源基因(CaMV35S,NOS,Cry1Ab/c)。结果表明,利用SYBR Green Ⅰ染料能结合双链DNA的特点,应用实时PCR技术可检测到转Cry1Ab/c基因抗虫水稻外源基因(CaMV35S,NOS,Cry1Ab/c)扩增所产生的荧光信号,通过扩增产物的熔解曲线能有效地区分特异性产物、非特异性产物以及引物二聚体。SYBR Green Ⅰ实时PCR技术是转基因成分检测的一种新方法。

Bt genetically modified rice was identified with SYBR Green Ⅰ real-time PCR assays and three pairs of specific primers to establish a new detection technique for GMO. The results showed that the SYBR Green Ⅰ could selectively combine the characteristic of double chain DNA and identify fluoresce signal produced by sequence amplification of specific target of Bt rice （CaMV35S,NOS,Cry1Ab/c）. The SYBR Green Ⅰreal-time PCR could availably differentiate special or non-special products and primer dimers by melting curve, which is a new method for GMO detection.