高效液相色谱法测定山豆根中红车轴草苷的含量

Determination of trifolirhizin in Sophora tonkinensis Gapnep.by HPLC

目的建立测定山豆根药材中红车轴苹苷的含量测定方法。方法色谱柱为Hypersil ODS2（250mm×4．6mm，5μm），流动相为乙腈0．1％磷酸水溶液，梯度洗脱，流速为1．0mL·min^-1，检测波长为308nm，柱温为25℃。结果红车轴草苷在0．4192～8．384扯g线性关系良好，相关系数r=0．9999，平均回收率为99．3％，RSD=1．6％。结论所用方法简便、准确、重复性好，首次建立了HPLC测定山豆根中红车轴草苷的含量，提供了更好的控制山豆根药材质量的方法。

Objective To establish a method for the determination of trifolirhizin in Sophora tonkinensis Gapnep.. Methods The separation was performed on a Hypersil ODS2 （250mm×4.6mm,5μm）. The mobile phase consisted of ACN-0.1%H3PO4 with a gradient elution. The flow rate was 1.0 mL·min^-1. The UV detection wavelength was 308 nm. Results The calibration curves of trifolirhizin were in good linearity at 0. 419 2-8.384μg （r=0.999 9）. The average recovery was 99.3% with RSD of 1.6%. Conclusion This method is simple, accurate and repeatable. The HPLC method to determine trifolirhizin in Sophora tonkinensis Gapnep. has been first established, which provides a better quality control method for Sophora tonkinensis Gapnep.