多巴胺D_2受体基因TaqIA多态性与酒依赖的关系

Association of TaqIA Polymorphism of Dopamine D_2 Receptor Gene and Alcohol Dependence

探讨中国汉族人群中多巴胺D2受体(DRD2)基因TaqIA多态性与酒依赖的相关性。采用聚合酶链式反应——限制性片断长度多态性(PCR-RFLP)技术,检测酒依赖组和对照组的DRD2基因TaqIA多态性的基因型和等位基因频率。采用汉密尔顿抑郁量表(HAMD)对酒依赖组和对照组进行评定,根据HAMD评分把酒依赖组分成酒依赖组(1)和酒依赖组(2),分析了两组DRD2基因TaqIA多态性的基因型和等位基因频率。结果为:①酒依赖组和对照组的DRD2基因TaqIA多态性的基因型和等位基因频率有显著性差异(前者携带A1等位基因的频率为0.614,后者携带A1等位基因的频率为0.314,P<0.05),携带A1等位基因者更易形成酒依赖(OR:3.475,P<0.05)。②酒依赖组和对照组的HAMD分值有显著性差异(前者为17.7 273±5.3 808,后者为8.1 142±3.8 378,P<0.05)。酒依赖组(1)和酒依赖组(2)的DRD2基因TaqIA多态性的基因型和等位基因频率无显著性差异。

Objective：To investigate the association of TaqlA polymorphism of DRD2 receptor gene with alcohol dependence in Chinese Han. Method ： Genotype and allele frequencies of TaqlA polymorphism of DRD2 receptor gene in dependence group）and control group were examined by polymerase chain reaction and restriction fragment length polymorphism （PCR- RFLP） technique. Both groups were performed with the Hamilton Rating Scale for depression （HAMD）. Dependence group was divided into dependence group 1 dependence group 2 by whether the HAMD scores 〉 20 or not. Result ： 1. The genotype and allele frequencies of DRD2 gene TaqlA polymorphism differed significantly between dependence group and control group. A1/A1 genotype and A1 allele frequency in dependence group was 0. 614, and 0.314 in control group （P 〈 0. 05 ）. A1 allele frequency was associated positively with alcohol dependence （OR：3. 475, P 〈0. 05 ）. 2. The HAMD scores were different significantly in dependence group（ 17. 7273±5. 3808 ） and control group（ 8.1142±3. 8378） , P 〈 0.05. The genotype and allele frequencies of DRD2 receptor gene TaqIA polymorphism did not differ significantly between dependence group （1） and dependence group （2）.