莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞凋亡

Morroniside Inhibits Hydrogen Peroxide-induced Apoptosis in SH-SY5Y Neuroblastoma Cells

目的研究莫诺苷抑制过氧化氢诱导的神经细胞凋亡作用和机制。方法培养的人神经母细胞瘤细胞系SH-SY5Y细胞加入莫诺苷1μmol/L、10μmol/L、100μmol/L预孵育24h,加入H2O2300～500μmol/L作用18h,检测神经细胞丙二醛(MDA)、线粒体膜电位(MMP)、细胞凋亡。结果1μmol/L、10μmol/L、100μmol/L莫诺苷显著抑制丙二醛增加,降低细胞膜电位,抑制细胞凋亡。结论莫诺苷能够通过抑制H2O2诱导的SH-SY5Y神经细胞脂质过氧化,降低细胞膜电位来抑制细胞凋亡。

Objective To investigate the effects of morroniside on hydrogen peroxide （H2O2）-induced apoptosis in SH-SY5Y neuroblastoma cells. Methods SH- SY5Y cells were pre-ineubated with morroniside （1, 10, and 100 μmol/L） for 24 h prior to exposure to H2O2 （300- 500 μmol/L） for 18 h. The content of malondialdehyde （MDA）, mitochondrial membrane potentials （MMP） and apoptosis ratio were determined. Results Pretreatment the cells with morroniside （1, 10 and 100 μmol/L） lowered the H2O2-induced MDA concentration and MMP, and inhibited the apoptosis. Conclusion Morroniside may protect the neurocyte from H2O2-induced apoptosis by lowered MDA and inhibits MMP.