促红细胞生成素对兔脊髓缺血再灌注损伤后核因子-κB表达的影响

Effect of Erythropoietin on Expression of Nuclear Factor-κB in Rabbit Spinal Cord Tissue after Ischemia-Reperfusion

目的探讨促红细胞生成素(EPO)对兔脊髓缺血再灌注损伤后神经功能及核因子-κB(NF-κB)表达的影响。方法建立兔脊髓缺血再灌注损伤模型,应用Tarlov评分法、免疫组化方法对各组动物后肢神经功能及脊髓组织NF-κB表达变化进行观察。结果促红细胞生成素治疗组神经功能恢复较缺血再灌注组明显提高(P<0.01),NF-κB表达明显低于缺血再灌注组(P<0.01)。结论EPO对缺血再灌注损伤的脊髓具有保护作用,其作用机制与抑制NF-κB表达有关。

Objective To explore the effects of erythropoietin （EPO） on the neural function recovery and the expressions of nuclear faetor-κB （NF-κB） in rabbit spinal cord after ischemia-reperfusion injury. Methods 24 rabbits were randomized into 3 groups： EPO treatment group, ischemia-reperfusion （I/R） group and sham group with 8 rabbits in each group. The rabbit model of spinal cord isehemia-reperfusion injury was established with Tetik＇s method. The changes of neural functional recovery of rabbits of 3 groups were observed through Tarlov＇s scale at different time points post-operation. The expressions of NF-κB were tested with immunohistochemistry. Results The grade of nerve function improved distinctly in EPO treatment group （P〈0.01）. The expression of NF-κB in EPO group was lower than that of I/R group （P〈0.01）. Conclusion EPO can significantly improve the motor function of hind limbs in rabbits after spinal cord ischemia-reperfusion injury, which might be related with inhibition of the expressions of NF- κB.