Livin在皮肤鳞状细胞癌中的表达及其与FHIT、Caspase-3相关性的初步研究

Expression of Livin protein and its relationship with Caspase-3, FHIT protion in skin squamous cell carcinoma

目的研究Livin在皮肤鳞状细胞癌（SCC）中的表达及其与脆性组氨酸三联体（FHIT）、半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）表达的相关性。方法应用免疫组织化学SP法检测Livin、FHIT、Caspase-3蛋白在48例SCC、20例慢性溃疡及12例正常皮肤组织中的表达。结果Livin在SCC的表达（33／48）明显高于慢性溃疡组织（3／20）及正常组织（0／12）（P〈0．01）；Caspase-3及FHIT在SCC中的表达明显低于正常皮肤和慢性溃疡组织（P〈0．01）；Livin、Caspase-3表达与肿瘤的分化程度、淋巴结转移差异有统计学意义（P〈0．05）。Livin、Caspase-3的表达与患者的年龄、性别以及肿瘤大小差异无统计学意义（P〉0．05）。SCC组织中Livin与Caspase-3（rs=-0．509，P：0．004）、FHIT（rs=-0．413，P=0．023）的表达呈负相关。结论Livin、FHIT、Caspase-3在SCC中的异常表达可能与SCC的发生、发展有关，并有可能成为SCC的预防和治疗的靶标。

Objective To study the expression of Livin and its relationship with Caspase-3, FHIT in skin squamous cell carcinoma. Methods Immunohistochemical assay was used to detect the expression of Livin, Caspase-3 and FHIT in 48 cases of sSCC, 20 cases of chronic ulcer and 12 cases of normal skin tissues. Results The rate of positive expression of Livin in SCC tissues was significantly higher than that in the chronic ulcer tissues and normal skin tissues （P 〈0.01）. The rate of positive expression of Caspase-3 and FHIT in SCC tissues was significantly lower than that in the chronic ulcer tissues and normal skin tissues （P 〈 0.01）.The expression of Livin and Caspase-3 had relationship with the lymphatic metastasis and histologic differentiation of SCC（P 〈0.05）. The expression of Livin and Caspase-3 had not relationship with age, sex, the sizes of SCC （P 〉0.05）. There was negative significant correlation between the expression of Livin and the expression of Caspase-3, FHIT. Conclusion There may be relationship between the abnormal expression of Livin and Caspase-3, FHIT and the occurrence, progression of skin squamous cell carcinomas. They may become a new method for preservation and treatment skin of squamous cell carcinomas.