Survivin与VEGF在结直肠肿瘤血管形成中的作用

The role of Survivin and VEGF in angiogenesis of colorectal tumor

目的探讨Survivin、血管内皮生长因子（VEGF）在结直肠肿瘤血管形成中的作用。方法对11例增生性息肉、23例低度异型增生腺瘤、20例高度异型增生腺瘤、29例腺瘤恶变组织和65例腺癌组织使用Survivin多克隆抗体、VEGF和CD34单克隆抗体、标准化的链霉菌抗生物素蛋白-过氧化物酶技术（SP法）对存档组织块切片进行免疫组化染色，并用MIRS-2000图像分析系统分析其阳性率及表达程度，并行微血管密度（MVD）计数。结果Survivin表达与VEGF表达及MVD密切相关，且呈明显正相关（r均为0．711，P〈0．01）。MVD与VEGF表达呈明显正相关（r=0．711，P〈0．01）。Survivin／VEGF双阳性组较阴性／阳性组及双阴性组MVD值显著增高（P均〈0．01），同时对于Survivin／VEGF阳性／阴性组，其MVD值与双阳性组差异无统计学意义（P〉0．05）。结论Survivin与VEGF表达密切相关，二者协同作用促进结直肠肿瘤血管生成；VEGF可能是结直肠癌中Survivin再表达的原因之一。

Objective To identify the role of survivin and VEGF in angiogenesis of colorectal tumor by investigating tissue expression of survivin, VEGF and CD34 in human colorectal tumors, Methods Immunohistochemical staining for the paraffin sections by using the polyclonal antibodies of survivin, monoclonal antibodies of VEGF and CD34, was performed by the standard avidin-biotin-peroxidase technique. The percentage of positive cell and quantitative analysis were made by image analysis system, which also helped to calculate the amount of MVD. Results The immunoreactivity of survivin significantly increased in the transition from adenoma with low dysplasia to adenoma with high dysplasia （P 〈0.01）. Similar changes in protein expression were observed for VEGF and MVD. The expression of survivin was closely related with that of VEGF. Both were positively correlated with MVD. Conclusion Suivivin promotes angiogenesis of colorectal tumor together with VEGF. VEGF may be the potential causes of reexpression of survivin during colorectal tumorigenesis.