生脉注射液在体外对精子运动能力和存活时间的影响

SHENG MAI ZHUSHEYE Improves the Viability and Movement Parameters of Human Sperm in Vitro

目的:观察生脉注射液对人精子运动参数和存活时间的影响。方法:将33例正常生育男性精液均分为两份,一份与生脉注射液+Hams-F10孵育,一份与Hams-F10共孵育。于0.5、1、2、4、8、12 h取样,检测精子存活率、并应用计算机辅助精子分析系统(CASA)测定精子的直线运动速度(VSL)、曲线运动速度(VCL)、平均路径速度(VAP)和头部侧摆幅度(ALH)等精子运动参数。结果:生脉组在孵育8 h时VCL明显高于Hams-F10组(P<0.05),12 h时生脉组VCL显著增高(P<0.01)。孵育4、8、12 h,生脉组VSL和VAP明显高于Hams-F10组(P<0.05)。孵育2、4、8、12 h时生脉组ALH明显高于Hams-F10组(P<0.05)。两份精液各时间段的精子死活比率相比,除12 h时有明显差异(P<0.05)外,其他各时间段无明显差异。结论:生脉注射液能提高精子的运动能力、延长精子的体外存活时间。

Objective ： To observe the effect of SHENG MAI ZHUSHEYE on the movement parameters and viability of human sperm in vitro. Methods： We collected sperm samples from 33 normal fertile men, divided each into two, and cultured them in vitro with SHENG MAI ZHUSHEYE ＋ Hams-F10 and Hams-F10 alone, respectively. Then we measured the straight line velocity （ VSL）, curvilinear velocity （VCL）, average path velocity （VAP） and the amplitude of lateral head displacement （ALH） of the sperm by computeraided semen analysis at 0.5, 1, 2, 4, 8 and 12 h. And the sperm viability was detected. Results ： VCL was significantly higher at 8 h （P 〈 0.05 ） and very significantly higher at 12 h （P 〈 0. 01 ） in the SHENG MAI ZHUSHEYE ＋ Hams-F10 group than in the Hams-F10 group. VSL, VAP and ALH were significantly increased in the former group at 4, 8 and 12 h as compared with the latter （P 〈 0.05）. The sperm viability was significantly decreased in the Hams-F10 group at 12 h （P 〈 0.05 ）. Conclusion： SHENG MAI ZHUSHEYE can improve sperm movement parameters and increase sperm viability in vitro.