多指标结合优化白疕消软膏药材提取工艺

Optimum of extraction process for crude drugs in Baibixiao qintment by combined multiple factors experiment analysis

目的:优化白疕消软膏药材提取工艺。方法:以大黄素、大黄酚含量测定与银屑病小鼠模型实验结果结合,采用L9(34)正交试验法对其关键步骤如煎煮时间(A)、煎煮次数(B)、醇沉时加醇量(C)、加水量(D)等条件进行优选。结果:4个因素对大黄中被测成分的影响顺序为B>D>A>C,对小鼠尾部鳞片表皮颗粒层形成的促进作用的影响顺序为B>D>C>A,其中因素B(煎煮次数)有显著性影响(P<0.05)。结论:最佳工艺为加药材量8倍的水煎煮3次,每次1.5h,浓缩液加2倍量乙醇使沉淀。

OBJECTIVE To optimize the extraction process of the crude medicine in Baibixiao qintment. METHODS By the emodin, the chrsophanol rhubarb content determination and psoriasis mouse model experiment were combined, L9 （3^4） orthogonal testing method was used to its essential step like watering quantity, the decoction time, the decoction times conditions for adding alcohol. RESULTS The influence factors were listed as B〉D〉A〉C and B〉D〉C〉A. Factor B （decoction times） had the significance influence（P〈0. 05）. CONCLUSION The optimum process of water volume 8 times of herbal weight was selected the extraction was conducted three time, 1.5 h each time.