外源一氧化氮供体硝普钠对干旱胁迫下小麦幼苗叶中ATP酶活性和膜脂过氧化的影响

Effects of Exogenous Nitric Oxide Donor Sodium Nitroprusside on ATPase Activity and Membrane Lipid Peroxidation in Wheat (Triticum aestivum L. cv. ‘Luohan 6’) Seedling Leaves under Drought Stress

研究外源一氧化氮(NO)供体硝普钠(SNP)对干旱胁迫下小麦幼苗叶片ATP酶活性和膜脂过氧化影响的结果表明,15%聚乙二醇-6000(PEG-6000)模拟的干旱胁迫下小麦幼苗叶中H+-ATP酶和Ca2+-ATP酶活性显著升高后迅速下降,硫代巴比妥酸反应产物(TBARS)和质量膜透性增加;0.1mmol·L-1SNP可提高干旱胁迫下小麦幼苗叶中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,降低超氧阴离子(O2-·)和过氧化氢(H2O2)水平,缓解膜脂过氧化,稳定生物膜的结构和功能,H+-ATP酶和Ca2+-ATP酶也可以保持更高的活性。

The effects of nitric oxide （NO） donor sodium nitroprusside （SNP） on ATPase activity and membrane lipid peroxidation in the wheat （Triticum aestivum cv. ‘Luohan 6＇） seedling leaves under drought stress were investigated. Results showed that the 15% PEG-6000 treatment apparently enhanced the H^＋-ATPase and Ca^2＋-ATPase activities before period of deterioration, accelerated the accumulation of thiobarbituric acid reac- tive substances （TBARS） and membrane permeability in wheat seedling leaves under drought stress. 0.1 mmol.L^-1 SNP could enhance the activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT）, lower O2^- production rate, H2O2 content and membrane lipid peroxidation, stabilize the structure and function of biomembranes, increase the activities H^＋-ATPase and Ca^2＋-ATPase.