期刊文献+

PDF1.2基因在转草酸氧化酶基因甘蓝型油菜中的表达 被引量:5

Expression of PDF1.2 in Oxalate Oxidase Transgenic Oilseed Rape (Brassica napus L.)
原文传递
导出
摘要 采用RT-PCR方法检测茉莉酸(JA)/乙烯(ET)依赖性信号途径中关键基因PDF1.2在转草酸氧化酶基因(OXO)油菜株系与未转化对照中的表达差异。结果表明,在转基因油菜不同株系中PDF1.2都有不同程度的上调表达,预示着转OXO油菜对菌核病的抗性增强可能与激活JA/ET依赖性信号途径有关。 The expressions of PDF1.2, a key gene in the jasmonic acid (JA)/ethylene (ET)-dependent signaling pathway, were measured in oxalate oxidase (OXO) transgenic and non-transgenic oilseed rapes (Brassica napus) by RT-PCR. The results showed that the expression of PDF1.2 was up-regulated in different OXO transgenic oilseed rape lines, which indicated that the mechanism of disease resistance to Sclerotinia sclerotiorum was related to the induction of the JA/ET-dependent signaling pathway.
出处 《植物生理学通讯》 CSCD 北大核心 2009年第5期479-482,共4页 Plant Physiology Communications
基金 国家自然科学基金(30671344、30800748) 中国博士后科学基金(20070411080)
关键词 甘蓝型油菜 菌核病 PDF1.2 转草酸氧化酶基因 oilseed rape (Brassica napus) Sclerotinia sclerotiorum PDF1.2
  • 相关文献

参考文献15

  • 1黄剑华,陆瑞菊,周志疆,王亦菲,孙月芳,周润梅.应用小孢子和花药培养技术筛选油菜抗菌核病材料[J].植物生理学通讯,2001,37(3):226-227. 被引量:13
  • 2冀瑞琴,董彩华,高荣村,刘越英,刘胜毅.甲基茉莉酸、苯丙噻重氮、草酸诱导油菜对菌核病的抗性[J].中国油料作物学报,2006,28(2):184-188. 被引量:14
  • 3刘胜毅,周必文,潘家荣.油菜对毒素草酸的吸收代谢与抗性机理[J].植物病理学报,1998,28(1):33-37. 被引量:29
  • 4de Bary A (1887). Comparative Morphology and Biology of the Fungi, Mycetozoa and Bacteria. Oxford: Clarendon Press, 1380-1388
  • 5Donaldson PA, Anderson T, Lane BG, Davidson AL, Simmonds DH (2001). Soybean plants expressing an active oligomeric oxalate oxidase from the wheat gf-2.8 (germin) gene are resistant to the oxalate-secreting pathogen Sclerotinia sclerotiorum. Physiol Mol Plant Pathol, 59:297-307
  • 6Dong X, Ji R, Guo X, Foster S J, Chen H, Dong C, Liu Y, HU Q, Liu S (2008). Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus). Planta, 228:331-340
  • 7Dunwell JM, Khuri S, Gane PJ (2000). Microbial relatives of the seed storage proteins of higher plants: conservation of structure and diversification of function during evolution of the cupin superfamily. Microbiol Mol Biol Rev, 64:153-179
  • 8Hammond-Kosack KE, Jones JDG (1996). Resistance gene-dependent plant defense responses. Plant Cell, 8:1773-1791
  • 9Hu X, Bidney DL, Yalpani N, Duvick JP, Crasta O, Folkerts O, Lu G (2003). Overexpression of a gene encoding hydrogen peroxide-generating oxalate oxidase gene evokes defense responses in sunflower. Plant Physiol, 133:1-12
  • 10Lamb C, Dixon RA (1997). The oxidative burst in plant disease resistance. Annu Rev Plant Physiol Plant Mol Biol, 48 : 251 -275

二级参考文献22

  • 1Rejane L,Guimaraes,Henrik U,et al.Oxalate production by Sclerotinia sclerotiorum deregulates guard cells during infection[J].Plant Physiology,2004,136:3703-3711.
  • 2Mucharromah E K,Kuc J.Oxalate and phosphate induce systemic resistance against disease caused by fungi bacteria and viruses in cucumber[J].Crop Protection,1991,10(4):265-270.
  • 3Liu S,Wang H,Zhang J,et al.In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorum[J].Plant Cell Reports,2005,24:133-144.
  • 4Liu S,Liu Z,Fitt B D L,et al.Resistance to Leptosphaeria maculans (phoma stem canker) in Brassica napus (oilseed rape) induced by L.biglobosa and chemical defence activators in field and controlled environments[J].Plant.Pathology,2006,55:401-412.
  • 5Young C S,Clarkson J P,Smith J A,et al.Environmental conditions influencing Sclerotinia sclerotiorum infection and disease development in lettuce[J].Plant Pathology,2004,53:387-397.
  • 6Hammerschmidt R.Induced disease resistance:how do induce plants stop pathogens?[J].Physiology and Molecular Plant Pathology,1999,55:77-84.
  • 7Ryals J A,Neuenschwander U H,Willits G M,et al.Systemic acquired resistance[J].Plant Cell,1996,8:1809-1819.
  • 8White R F.Short communications:acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco[J].Virology,1979,99:410-412.
  • 9Metraux J P.Systemic acquired resistance and salicylic acid:current state of knowledge[J].Europe Journal of Plant Pathology,2001,107:13-18.
  • 10Yanar Y,Miller S.Effect of treating pepper with benzothiadiazole derivative and non-pathogenic xanthomonas campestris pathovars on the level of disease caused by Sclerotinia sclerotiorum[J].Plant Pathology Journal,2004,3(1):52-55.

共引文献51

同被引文献55

引证文献5

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部