谷氨酰tRNA合成酶转基因拟南芥的生长特性分析

Phenotypic analysis of glutamyl-tRNA synthetase in transgenic Arabidopsis thaliana and researches on abiotic resistance of transgenic plants

以不同独立株系的拟南芥谷氨酰tRNA合成酶(Arabidopsis glutamyl tRNA syn-thetase,简称AtGluRS)过量表达的三种纯合株系(GS3、GS4、GS6)及抑制表达的三种纯合株系(GAS2、GAS3、GAS7)为实验材料,分析其生长情况和表型。表型分析结果表明,拟南芥谷氨酰tRNA合成酶过量表达植株表现出开花晚、基叶多、茎生长快等与对照和AtGluRS抑制表达植株不同的特点。此外,用氯化钠(NaCl)模拟盐胁迫环境,对转基因植物的萌发率和生长情况进行分析,发现谷氨酰tRNA合成酶的过量表达增强了植物对盐胁迫的耐受能力。因此,拟南芥谷氨酰tRNA合成酶影响植物表型并参与植物抗逆过程。

The phenotype of independent homozygous transgenic strains of glutamyl tRNA synthetase in Arabidopsis （Arabidopsis glutamyl tRNA synthetase, or AtGIuRS） has been analyzed, including three over-expressed （GS-3, GS4, GS-6） and three inhibit-expressed （GAS2, GAS3, GAST）. It was showed that comparing to inhibit-expressed strains, plants with over-expressed AtGluRS were flowering later, growing faster and having more rosette leaves. In addition, the germination rate and growth analysis of AtGluRS transgenic plants under salt stress with sodium chloride （NaCl） reflected that over-expressing AtGIuRS could enhance the resistance of plants to salt stress. Therefore, glutamyl tRNA synthetase in Arabidopsis not only affected plants phenotype, but also was involved in the process of abiotic stress tolerance.