应激对机体脑体功能的影响及其生物学机制初探

EFFECTS OF STRESS ON THE COGNITIVE FUNCTION AND PHYSICAL FITNESS AND ITS BIOLOGICAL MECHANISMS

目的:探讨应激对机体脑体功能的损伤效应及其生物学机制,为应激损伤防护措施的制订提供科学依据。方法:采用束缚应激模型,观察模型动物认知功能、体能、海马LTP、血浆糖皮质激素、心电图、心肌组织结构等指标的变化。结果:应激动物学习记忆能力和运动耐力明显下降,血浆糖皮质激素水平显著升高,海马LTP诱发受到抑制,心电图异常改变,心功能紊乱,心肌组织结构出现病理损伤,心肌细胞凋亡率增加,心肌组织Hsp70表达水平随应激强度增加而逐渐降低。结论:应激诱导机体神经-内分泌功能紊乱,进而导致机体脑体功能损伤。

Aim： To study the effects of stress on the cognitive function and physical fitness and its biological mechanisms, provide scientific basis for seeking protective measures to reduce stress-induced damage. Methods： The model of restraint stress was adopted in our experiment. Step- down test and exhaustive swimming test were used to measure the leaming and memory function and physical fitness in mice, respectively. The contents of GC in plasma, hippocampus LTP, ECG, myocardial ultra-structure, cell apoptosis rate and the level of Hsp70 expression of myocardium in rats were detected. Results： Compared with control group, the impairment of learning and memory function and decline of exercise tolerance were observed in restraint stress group. The elevation in plasma GC levels, ECG abnormality, and cell apoptosis rate were also observed under restraint stress. Furthermore, myocardial structure was damaged, and myocardial Hsp70 expression and hippocampas LTP were suppressed in restraint stress group than those in the control. Conclusion： Stress may cause the neuro-endocrine dysfunction and homeostasis disorder, and then, lead to the cognitive function and physical fitness damage consequently.