急性低氧暴露小鼠外周血代谢组变化分析

EFFECTS OF ACUTE HYPOXIA ON PLASMA METABOLOME IN MICE

目的:探讨急性低氧对小鼠外周血代谢组的影响。方法:将14只小鼠随机分为正常组和低氧组。用基础饲料喂养2周后,将低氧组减压至6 000 m模拟高度停留8 h,实验结束后,采集静脉血制备血浆待测。在核磁共振波谱仪进行1HNMR检测,采用模式识别分析方法处理数据。结果:与正常组相比,低氧组乳酸含量明显增加,肉碱水平明显降低;脂类、丙氨酸、丙酮酸、谷氨酰胺、胆碱、牛磺酸和葡萄糖含量升高,缬氨酸、β-羟丁酸、谷氨酸、甘油、甘氨酸和丝氨酸含量下降。结论:急性低氧暴露使小鼠血浆碳水化合物、脂肪代谢和氨基酸代谢谱发生变化,表明低氧后能量代谢以及相关物质含量发生改变。

Aim： To explore the metabolic effects of acute hypoxia on mice plasma. Methods： Fourteen mice were randomly divided into two groups： control and hypoxia group. The mice of hypoxia group were exposed to a simulated altitude of 6 000 meters for 8 hours. Nuclear magnetic resonance spectrometer was used to identify the metabolic changes after acute hypoxia. Results： Compared with control, the most notable metabolic changes in plasma were as follows： camitine decreased remarkably and lactate increased significantly after acute hypoxia exposure. Decreases in levels of β-HB, ethanol glycerol, glutamate, glycine and serine, and increased levels of lipids and pyruvate, alanine, taurine, choline, glucose, and glutamine were also observed in hypoxia group. Conclusion： Significant changes in the plasma carbohydrate, lipid and amino acid profiles were observed following acute hypoxia, suggesting a hypoxia-induced alteration in energy and related substances metabolism.