摘要
本研究利用体外淋巴细胞培养和噻唑蓝（ＭＴＴ）比色分析法，检测了两种浓度多巴胺（ＤＡ，１０－５和１０－６ｍｏｌ／Ｌ）对大鼠肠系膜淋巴结细胞由刀豆素Ａ（ＣｏｎＡ）诱导的增殖反应的影响；并运用高效液相色谱（电化学检测法（ＨＰＬＣ－ＥＣＤ），测定了大鼠在注射抗原绵羊红细胞（ＳＲＢＣ）后的２～７天，胸腺和脾脏中ＤＡ含量的变化。结果说明：（１）１０－５和１０－６ｍｏｌ／ＬＤＡ都能显著增强Ｔ细胞增殖。（２）大鼠在体液免疫应答的第６天，胸腺和脾脏内的ＤＡ含量显著增多，其它时期ＤＡ含量虽有变化，但无显著意义。以上结果提示，外周ＤＡ既可增强细胞免疫功能。
In this study, we determined, in vitro, the effect of dopamine (DA, 10-5 and 10-6 mol/L) on the proliferation of the cells in the rat mesenteric lymph nodes induced by concanavalin (Con A) and sought the change of content of DA in the thymus and spleen of rats immunized with sheep red blood cells (SRBC) during day 2-7 post-immunization by the aid of the high-performance liquid chromatography with electrochemical detection (HPLC-ECD). The results are as follows: DA at 10-5 and 10-6 mol/L significantly enhanced the T lymphocyte proliferation. On day 6 of humoral immune response, DA content in the thymus and spleen markedly increased compared with the saline control. In the other period of the antibody response, although DA content in these lymphoid organs had some changes, there was no marked significance. Above results show that on the one hand, DA can enhance the function of the cellular immunity and on the other hand, the humoral immunity can affect the content of DA in the lymphoid organs, which suggests that an interaction exists between the DA and immune system.
出处
《南通医学院学报》
1998年第1期9-10,共2页
ACTA Academiae Medicinae Nantong
关键词
多巴胺
细胞免疫
免疫功能
dopamine cellular immunity humoral immunity immunoregulation
