摘要
目的:应用蛋白质组学方法分析病理性心肌肥大心肌细胞蛋白表达特征。方法:雄性SD大鼠16只,分为2组(n=8):两肾一夹组(2K1C)和假手术组(SO),术后饲养8周,使用普通多普勒和组织多普勒鉴定动物模型,然后提取心肌总蛋白,应用二维凝胶电泳技术,建立分辨率高和重复性良好的凝胶图像,质谱(MALDI-TOF-MS)鉴定差异蛋白点,与网络数据库进行匹配,并对鉴定蛋白进行分类。结果:两肾一夹大鼠心肌细胞有21个蛋白点表现出显著增加或减少,经数据库匹配,获得14种差异表达的蛋白质。结论:两肾一夹大鼠心肌出现一些差异表达蛋白质,它们可能在病理性心肌肥大的发生中起重要作用。
To identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation. Methods： 16 male SD rats were separated into 2 groups（ n = 8） ： 2 kidney 1 Clip group（2K1C） and sham operation group（SO）. The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）. Results： Overall, 21 protein spots showed significant difference, and 14 out of which were identified. Conclusion： Kidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第2期237-242,共6页
Chinese Journal of Applied Physiology
基金
江苏省体育局基金资助项目(TY6109)
关键词
蛋白质组
二维电泳
质谱
心肌肥大
肾动脉缩窄
proteomics
two-dimensional electrophoresis
mass spectrometry
cardiac hypertrophy
kidney artery coarctation
