链霉素耳中毒豚鼠耳蜗螺旋神经节iNOS、AChE表达及丹参的保护作用

iNOS AND AChE EXPRESSION ON GUINEA PIGS COCHLEA SPIRAL GANGLION INDUCED BY STREPTOMYCIN AND ATTENUATION BY SALVIA MILTIORRHIZA INJECTION

目的:探讨链霉素(SM)耳中毒过程中豚鼠耳蜗螺旋神经节诱生型一氧化氮合酶(iNOS)与乙酰胆碱酯酶(AChE)的表达及二者之间的相关性,以及丹参(DS)的保护作用。方法:32只豚鼠随机分为4组:正常对照组、SM组、DS+SM组、DS组。应用免疫组化及图像分析技术检测iNOS和AChE表达及进行灰度值分析,结合听性脑干反应(ABR)测试监测耳毒性损伤的发生。结果:用药10 d后,SM组ABR阈值明显高于正常对照组,差异显著(P<0.01),而DS+SM联合用药组ABR阈值虽然高于正常对照组,但是与SM组相比明显降低(P<0.01)。免疫组化结果表明,SM组iNOS及AChE在螺旋神经节的表达明显高于正常对照组(P<0.01),且二者表达呈正相关;DS+SM联合用药组iNOS及AChE在螺旋神经节的表达低于SM组(P<0.05)。结论:SM耳中毒时ABR阈值升高,iNOS及AChE表达增强,而且具有一定的相关性;DS能够拮抗SM所致这一改变,具有保护作用。

To study the expression of iNOS and AChE on ginea pigs cochlea spiral ganglion induced by streptomycin（SM） and attenuation by salvia miltiorrhiza injection（Chinese Traditional medicine-dansen DS）. Methods： 32 guinea pigs were divided into 4 groups randomly（ n = 8） ： control group , SM group, DS ＋ SM group, DS group. SABC immunohistochemical staining and image quantitative analysis technique were used to observe the expression of iNOS and AChE, as well as grey value analysis, and ABR measurements were used to observe ototoxicity. Results： After 10 days with drugs, the ABR threshold value of SM increased more significantly than that of the control（ P 〈 0.01 ）, while the ABR threshold value of DS ＋ SM co-treatment increased than the control group, but lower than that of SM group（ P 〈 0.01）. The results of immunohistochemical staining implied the expression of iNOS and AChE in SG of SM group were higher than that of control group, and had positive correlate. Conclusion： The ABR threshold value increases and the expression of iNOS and AChE strengthen on SM ototoxicity, and has some correlation. DS can attenuate the ototoxicity induced by SM, and has protective function.