吴茱萸次碱对高血压大鼠胸主动脉血管肽酶C表达的影响研究

Effect of Rutaecarpine on the Expression of Angiotensin C in Thoracic Artery of Hypertensive Rats

目的:研究吴茱萸次碱对高血压大鼠胸主动脉血管肽酶C表达的影响。方法:应用两肾一夹高血压(2K1C)模型大鼠作为研究对象,以夹尾法测量血压。手术后10周分别给予氯沙坦(20mg·kg-1.d-1)和吴茱萸次碱(10、40mg·kg-1.d-1)干预治疗4周,观察每周血压变化。分析主动脉形态学结构变化;通过蛋白印迹法测定主动脉中血管肽酶C的含量。结果:治疗组吴茱萸次碱ig4周后,动脉收缩压显著降低。同模型组比较,治疗组大鼠胸主动脉血管管腔内径显著扩大,膜厚度变薄,血浆和动脉中血管紧张素Ⅱ显著降低,血管肽酶C的表达增强。结论:吴茱萸次碱能够有效降低血压、改善血管重构,其机制可能与表达增强的血管肽酶C介导的血管紧张素Ⅱ的灭活和激肽释放酶的激活有关。

OBJECTIVE： To explore the effect of rutaecarpine（Rut） on the expression of angiotensin C in the thoracic artery of hypertensive rats. METHODS： Two-kidney and one- clip goldhlatt （2KIC） hypertensive model rats were enrolled in our study.The blood pressure of the model rats was measured by tail- clipping method. The model rats were assigned to receive losartan（20mg·kg^-1·d^-1） or Rut（20mg·kg^-1·d^-1）with their blood pressure was followed every week. The morphology of aorta was analyzed and the content of angiotensin C in the thoracic artery was detected by Western blotting. RESULTS： After treatment with Rut for 4 weeks, the systolic arterial pressure（SAP） in the treatment group decreased sig- nificantly; as compared with model group, the luminal diameter dilated significantly, the medium thickness of the artery de- creased, angiotensin H level in plasma and arteries decreased significantly, while the expression of angiotensin C increased significantly. CONCLUSION： Rut can effectively decrease blood pressure and improve arterial remodeling in model rats. The mechanism might be associated to the deactivation of angiotensin Ⅱ and the activation of kallikrein medicated by the enhanced expression of angiotensin C.