五龙颗粒对变应性鼻炎模型大鼠鼻黏膜IL-3 mRNA表达的影响研究

Effect of Wulong Granules on the Expression of IL-3 mRNA of Nasal Mucosa in Rats with Allergic Rhinitis

目的:研究五龙颗粒对变应性鼻炎模型大鼠白细胞介素-3(IL-3)mRNA表达的影响,探讨其治疗变应性鼻炎的机制。方法:以卵清蛋白为变应原复制变应性鼻炎模型大鼠,随机分为模型(AR)组、五龙颗粒组和息斯敏组。取3组和健康大鼠(空白组)血清进行组胺检测;取大鼠鼻黏膜作苏木素-伊红染色,并采用逆转录-聚合酶链反应(RT-PCR)检测法对各组大鼠鼻黏膜组织中IL-3mRNA的表达水平进行相对定量比较。结果:IL-3mRNA在4组大鼠鼻黏膜中均有表达,其中AR组的表达水平为11.85±0.82,显著高于空白组(P<0.01);与AR组比较,五龙颗粒及息斯敏组大鼠鼻黏膜IL-3mRNA的表达显著下降(P<0.01)。结论:五龙颗粒能降低模型大鼠鼻黏膜IL-3mRNA的表达水平,可为临床合理用药提供理论基础。

OBJECTIVE ： To study the effect of Wulong granules on expression of IL - 3 mRNA of nasal mucosa in allergic rhinitis（AR） model rats and to discuss the mechanism for Wulong granules to treat AR. METHODS： AR model was duplicated in rats using ovoalbumin and the model rats were randomly assigned to AR model group, Wulong granules group and astemizol group with blank group as control. Serum histamine levels in the 4 groups were detected; the nasal mucosa was taken from rats for HE staining, and the expression of IL - 3 mRNA in the nasal mucosa were quantitatively detected by RT - PCR. RE- SULTS ： Positive IL - 3 mRNA expression of the nasal mucosa was noted in all 4 groups, which was significantly higher in AR group （11.85± 0.82） than in blank group （P〈0.01） . However, as compared with AR group, the IL-3 mRNA expression levels in both Wulong granules group and astemizole group were significantly down- regulated（P〈 0.01） .CONCLUSION： Wulong granvues can decrease the expression of IL- 3 mRNA in the nasal mucosa in allergic rhinitis rats, and the results serve as a theoretic basis for clinical rational drug use.