正交试验优选新疆苦巴旦杏仁中苦杏仁苷提取工艺

Optimization of Extract Technology of Amygdalin in Amygdala amara from Xinjiang by Orthogonal Experiment

目的:优选新疆苦巴旦杏仁中苦杏仁苷的提取工艺。方法:以苦杏仁苷含量为定量指标,通过单因素试验优选出的微波灭酶时间、苦巴旦杏仁细粉与石油醚比例、脱脂粕与95%乙醇的比例为考察因素,用正交试验优选最佳提取工艺。结果:最佳提取工艺为微波灭酶3.5min,苦巴旦杏仁细粉与石油醚比例为1∶6进行脱脂,脱脂粕与95%乙醇为1∶30进行纯化,在此条件下苦杏仁苷的含量为3.21%。结论:该提取工艺合理、简单、可行,可为大生产提供理论依据。

OBJECTIVE： To optimize the extract technology of amygdalin in Amygdala amara from Xinjiang. METHODS： Orthogonal experiment was carried out to optimize the extraction technology with the content of amygdalin as quantitative index, and with the duration of enzyme - deactivation by microwave, the ratio of Amygdala amara fine powder to petroleum benzin, and the ratio of defatted meal to ethanol as factors. RESULTS： The optimum extraction conditions were as follows： the duration of enzyme - deactivation by microwave was 3.5 min; the ratio of Amygdala amara fine powder to petroleum benzin was 1 ： 6,and the ratio of defatted meal to 95% ethanol was 1 ： 30. Under the above conditions, the content of amygdaloside reached 3.21%. CONCLUSION： The extraction technology was proved to be reasonable, simple and feasible, and it provides theoretical basis for large production.