HPLC法测定仙桃草中木犀草素的含量

Determination of Luteolin in Veronica peregrina by HPLC

目的:建立以高效液相色谱法测定仙桃草中木犀草素含量的方法。方法:色谱柱为Diamonsil-C18(250mm×4.6mm,5μm),流动相为甲醇-乙腈-水-磷酸(48∶5∶47∶0.4),流速为1mL·min-1,检测波长为254nm。结果:木犀草素进样量在0.1004～0.9036μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为97.5%,RSD=1.21%(n=9)。结论:本法简便、准确、灵敏,可用于仙桃草药材的质量控制。

OBJECTIVE： To determine the content of luteolin in Veronica peregrina by HPLC. METHODS： The chromatographic separation was performed on Diamonsil - C18（250mm×4.6mm.5μm） and the mobile phase consisted of methanol - acetonitrile - water - phosphoric acid（48 ： 5 ： 47 ： 0.4） at a flow rate of 1 mL ~ min 1 and with a detective wave- length of 254 nm. RESULTS： The linear range of luteolin was 0.100 4--0.903 6 μg（r = 0.999 9） with an average recovery rate of 97.5% （RSD = 1.21%, n = 9）.CONCLUSION： The method was proved to be simple, accurate and sensitive, and suitable for the quality control of luteolin in Veronica peregrina .