不同浓度甲醇对决明子中5种蒽醌类成分含量的影响考察

Effects of Different Concentration of Methanol on 5 Anthraquinone Constituents in Semen Cassiae

目的:考察不同浓度甲醇对决明子中5种蒽醌类成分含量的影响。方法:用不同浓度(95%、80%、30%)甲醇溶液提取决明子药材,采用高效液相色谱法测定药材中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量。结果:不同浓度甲醇溶液提取的决明子药材中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量范围分别为0.0010～0.0181、0.0104～0.0637、0.0209～0.2535、0.0031～0.9157、0.0051～0.1263mg·g-1;平均回收率范围为96.34%～97.16%,RSD均小于3%(n=5)。结论:不同浓度甲醇提取的决明子药材中5种蒽醌类化合物含量差异大;以95%甲醇提取效果最佳,30%甲醇提取效果最差。

OBJECTIVE： To investigate the effects of different concentration of methanol on 5 anthraquinone constituents in Semen Cassiae .METHODS： Semen Cassiae was extracted with different concentration of methanol （95%, 80% and 30% ）. The contents of aloe - emodin, rhein, emodin, chrysophanol and physcione in Semen Cassiae were determined by HPLC. RESULTS： The contents of aloe- emodin, rhein, emodin, chrysophanol and physcione were 0.001 0 --0.018 1 mg · g^-1, 0.010 4--0.0637mg-g 1,0.020 9--0.2535mg＇g--1,0.0031--0.9157mg＇g land0.0051-0.1263mg·g^-1,respectively, The average recoveries were 96.34%--97.16% and the RSDs of all were less than 3% （n ： 6） .CONCLUSION： There were great differences in the contents of the 5 anthraquinone constituents in Semen Cassiae extracted with different concentration of methanol, and a best extraction efficacy was achieved for Semen Cassiae extracted with 95% methanol while the worst extraction efficacy was noted if extracted with 30% methanol.