764-3对不同类型慢性肺动脉高压大鼠右心室肥厚胶原的影响

Effect of 764-3 on Ventricular Collagen Deposition in Pulmonary Hypertension Induced by Chronic Hypoxia and Monocrotaline in Rats

观察胶原合成抑制剂７６４－３对低压缺氧和野百合碱（ＭＣＴ）引起的肺动脉高压大鼠右心室胶原的影响。方法：将雄性Ｗｉｓｔａｒ大鼠随机分为１０组。低压缺氧给药组大鼠皮下注射 ７６４－ ３２０ｍｇ／ｋｇ，每日 １次； ＭＣＴ治疗组大鼠皮下注射 ７６４－３４０ｍｇ／ｋｇ，每日 １次。实验终点测定血流动力学及生化指标。结果：缺氧２周，４周及ＭＣＴ大鼠肺动脉压升高，右心室胶原含量明显增加；７６４－３能降低缺氧及 ＭＣＴ大鼠肺动脉压，减少缺氧２周及 ＭＣＴ大鼠右室胶原含量（μｇ），分别由７６．３±１３．３降至５９．４±１１．４（Ｐ＜０．０１），２９３．４±７３．９降至２１０．１±３７．６（Ｐ＜０．０１）。结论：７６４－３对缺氧性肺动脉高压大鼠右室胶原沉积仅有部分预防作用，对ＭＣＴ大鼠的右室肌胶原沉积有治疗作用。

To observe the effect of 764-3 on ventricular collagen deposition in pulmonary hypertension induced by chronic hypoxia or monocrotaline (MCT) in rats. Methods: Wistar rats were divided into 10 groups. The hypobaric hypoxia group received 764-3 20mg/kg once daily subcutaneously and the MCT group treated with 764-3 40mg/kg once daily. The hemodynamic and biochemical parameters were measured. Results: At the time of 2 weeks and 4 weeks after treated with hypoxia or MCT, the pulmonary arterial pressure (PAP) of rats raised significantly and the right ventricular collagen content (RVCC) increased markedly. 764-3 could reduce the raised PAP induced either by hypoxia or MCT. It could lower the elevated RVCC of hypobaric hypoxia group at 2 weeks from 76.3± 13.3 to 59.4±11.4 (P<0. 01), and the RVCC of MCT group lowered from 293.4±73.9 to 210.1± 37.6(P<0.01), Conclusion: 764-3 could partially prevent right ventricular collagen deposition induced by hypox ia and has therapeutic effect on that induced by MCT.